Center of Biotechnology in Reproduction (CEBIOR-BIOREN), Universidad de La Frontera, Temuco, Chile.
Center for Excellence in Morphological and Surgical Studies (CEMyQ), Universidad de La Frontera, Temuco, Chile.
Biomed Res Int. 2018 Nov 25;2018:5479057. doi: 10.1155/2018/5479057. eCollection 2018.
Obesity has adverse effects on male fertility and usually is diagnosed with a prevalence of vitamin D deficiency (VD). Discussion on the impact of obesity/VD on sperm function has been limited. This study analyzed the effects of diet-induced obesity/VD on viability and plasma membrane integrity (PMI), superoxide anion (O ) level, and DNA fragmentation (DNA) in sperm Sprague-Dawley rats. The males were randomized into four groups and fed for a period of 12 weeks: G1: control diet with vitamin D (C/VD), G2: control diet without vitamin D (C/VD), G3: high-fat diet with vitamin D (HF/VD), and G4: high-fat diet without vitamin D (HF/VD). Sperm function parameters were analyzed by flow cytometry. PMI percentages and O levels were not affected by any of the diets. DNA fragmentation was increasing significantly (p<0.05) in the spermatozoa of animals with diets vitamin D deficient (G2) and diet-induced obesity (G4). Our results allow us to point out that diet-induced obesity and VD produce greater damage in DNA sperm of rats. The use of nutraceuticals containing vitamin D could be reducing the risk of fragmentation of DNA in spermatozoa.
肥胖对男性生育力有不良影响，通常通过维生素 D 缺乏症（VD）的患病率来诊断。关于肥胖/VD 对精子功能的影响的讨论一直受到限制。本研究分析了饮食诱导的肥胖/VD 对精子活力和质膜完整性（PMI）、超氧阴离子（O ）水平和 DNA 碎片化（DNA）的影响。雄性大鼠随机分为四组，喂养 12 周：G1：含维生素 D 的对照饮食（C/VD），G2：不含维生素 D 的对照饮食（C/VD），G3：含维生素 D 的高脂肪饮食（HF/VD）和 G4：不含维生素 D 的高脂肪饮食（HF/VD）。通过流式细胞术分析精子功能参数。PMI 百分比和 O 水平不受任何饮食的影响。维生素 D 缺乏（G2）和饮食诱导肥胖（G4）的动物的精子 DNA 碎片化显著增加（p<0.05）。我们的研究结果表明，饮食诱导的肥胖和 VD 会导致大鼠精子 DNA 受到更大的损伤。使用含有维生素 D 的营养保健品可能会降低精子 DNA 碎片化的风险。
