通过寡核苷酸连接的金核-卫星纳米结构用于检测谷胱甘肽的 LSPR 散射光谱。

Gold core-satellite nanostructure linked by oligonucleotides for detection of glutathione with LSPR scattering spectrum.

机构信息

School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255000, China.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210000, China.

出版信息

Talanta. 2019 Feb 1;193:123-127. doi: 10.1016/j.talanta.2018.09.096. Epub 2018 Sep 26.

DOI:10.1016/j.talanta.2018.09.096

PMID:30368280

Abstract

We demonstrated a sensitive method for detection of glutathione (GSH) based on LSPR scattering spectrum using gold core-satellite nanostructure linked by T-Hg-T base pair. The core-satellite assembly caused coupling between plasmonic nanoparticles, which inducing distinct change of LSPR peak wavelength. As the interaction between Hg and GSH, the core-satellite nanostructure would be disassembled, which accompanied with spectral blue-shift of the scattering spectrum. By using this method, GSH could be quantitatively detected, and the detection limits can reach to 0.1 µM.

摘要

我们展示了一种基于金核-卫星纳米结构通过 T-Hg-T 碱基对连接的 LSPR 散射光谱检测谷胱甘肽 (GSH) 的灵敏方法。核-卫星组装导致等离子体纳米粒子之间的耦合，从而导致 LSPR 峰波长的明显变化。由于 Hg 与 GSH 之间的相互作用，核-卫星纳米结构会被拆开，同时散射光谱发生蓝移。通过使用这种方法，可以对 GSH 进行定量检测，检测限可达 0.1 µM。