German Natalija, Ramanaviciene Almira, Ramanavicius Arunas
Department of Immunology, State Research Institute Centre for Innovative Medicine, Santariskiu 5, LT-08406 Vilnius, Lithuania.
NanoTechnas-Center of Nanotechnology and Materials Science, Faculty of Chemistry and Geosciences, Vilnius University, LT-03225 Vilnius, Lithuania.
Polymers (Basel). 2020 Dec 17;12(12):3026. doi: 10.3390/polym12123026.
Nanocomposites based on two conducting polymers, polyaniline (PANI) and polypyrrole (Ppy), with embedded glucose oxidase (GOx) and 6 nm size gold nanoparticles (AuNPs) or gold-nanoclusters formed from chloroaurate ions (AuCl), were synthesized by enzyme-assisted polymerization. Charge (electron) transfer in systems based on PANI/AuNPs-GOx, PANI/AuNPs-GOx, Ppy/AuNPs-GOx and Ppy/AuNPs-GOx nanocomposites was investigated. Cyclic voltammetry (CV)-based investigations showed that the reported polymer nanocomposites are able to facilitate electron transfer from enzyme to the graphite rod (GR) electrode. Significantly higher anodic current and well-defined red-ox peaks were observed at a scan rate of 0.10 V s. Logarithmic function of anodic current (log ), which was determined by CV-based experiments performed with glucose, was proportional to the logarithmic function of a scan rate (log ) in the range of 0.699-2.48 mV s, and it indicates that diffusion-controlled electrochemical processes were limiting the kinetics of the analytical signal. The most efficient nanocomposite structure for the design of the reported glucose biosensor was based on two-day formed Ppy/AuNPs-GOx nanocomposites. GR/Ppy/AuNPs-GOx was characterized by the linear dependence of the analytical signal on glucose concentration in the range from 0.1 to 0.70 mmol L, the sensitivity of 4.31 mA mM cm, the limit of detection of 0.10 mmol L and the half-life period of 19 days.
基于两种导电聚合物聚苯胺（PANI）和聚吡咯（Ppy），嵌入葡萄糖氧化酶（GOx）以及6纳米尺寸的金纳米颗粒（AuNPs）或由氯金酸盐离子（AuCl）形成的金纳米团簇，通过酶辅助聚合合成了纳米复合材料。研究了基于聚苯胺/金纳米颗粒 - 葡萄糖氧化酶（PANI/AuNPs-GOx）、聚苯胺/金纳米颗粒 - 葡萄糖氧化酶（PANI/AuNPs-GOx）、聚吡咯/金纳米颗粒 - 葡萄糖氧化酶（Ppy/AuNPs-GOx）和聚吡咯/金纳米颗粒 - 葡萄糖氧化酶（Ppy/AuNPs-GOx）纳米复合材料体系中的电荷（电子）转移。基于循环伏安法（CV）的研究表明，所报道的聚合物纳米复合材料能够促进电子从酶转移到石墨棒（GR）电极。在扫描速率为0.10 V/s时观察到显著更高的阳极电流和明确的氧化还原峰。通过用葡萄糖进行的基于CV的实验测定的阳极电流对数函数（log ）与扫描速率对数函数（log ）在0.699 - 2.48 mV/s范围内成正比，这表明扩散控制的电化学过程限制了分析信号的动力学。所报道的葡萄糖生物传感器设计中最有效的纳米复合结构基于两天形成的聚吡咯/金纳米颗粒 - 葡萄糖氧化酶（Ppy/AuNPs-GOx）纳米复合材料。石墨棒/聚吡咯/金纳米颗粒 - 葡萄糖氧化酶（GR/Ppy/AuNPs-GOx）的特征在于分析信号与葡萄糖浓度在0.1至0.70 mmol/L范围内呈线性关系，灵敏度为4.31 mA mM cm，检测限为0.10 mmol/L，半衰期为19天。
