Mattioli Cristiano Campos, Takata Rodrigo, de Oliveira Paes Leme Fabiola, Costa Deliane Cristina, Luz Ronald Kennedy
Escola de Veterinária, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, n° 6627, Belo Horizonte, CEP 30161-970, Brazil.
Unidade de Pesquisa e Reprodução de Peixes, Fundação Instituto de Pesca do Estado do Rio de Janeiro, Av. Presidente Vargas, 197, Parque de Exposições, Niterói, CEP 28540-000, Brazil.
Fish Physiol Biochem. 2019 Feb;45(1):455-467. doi: 10.1007/s10695-018-0576-z. Epub 2018 Oct 27.
The present study aimed to evaluate the physiological and metabolic stress responses of juvenile Lophiosilurus alexandri submitted to an air exposure test. The subjects consisted of 72 juveniles. Blood samples were taken at: 0 h-fish not exposed to air; 0.5 h-fish shortly after exposure to air for 30 min (prior to returning to the tank); 1.5 h (90 min), 24, 48, and 96 h after the initiation of exposure to air for 30 min. After 96 h, survivorship was 100%. Cortisol and glucose levels were higher at 0.5 h, returning to baseline at 48 and 24 h, respectively. Lactate dehydrogenase levels were highest at 1.5 h after exposure to air, returning to normal values in 24 h. Several changes were recorded in gasometric blood values and electrolytes. With regard to hematology and blood chemistry, exposure to air did not affect globular volume and AST throughout the 96 h of the experiment. The values for alkaline phosphatase were highest at 0, 1.5, and 24 h. Total protein was similar between 0 and 1.5 h and lowest at 96 h, while ALT was highest at 0.5 h. Leukocytes were highest at 0.5, 1.5, 48, and 96 h, while erythrocytes were highest at 96 h. After 96 h, juvenile L. alexandri were able to reestablish the main indicators of stress (cortisol, glucose and lactate dehydrogenase), while other indicators (hematological, biochemical, and gasometric) exhibited compensatory variation for normal physiological re-establishment.
本研究旨在评估暴露于空气中的亚历山大低冠鲶幼鱼的生理和代谢应激反应。实验对象为72尾幼鱼。在以下时间点采集血样：0小时——未暴露于空气中的鱼；0.5小时——鱼暴露于空气中30分钟后不久（返回水箱之前）；暴露于空气中30分钟开始后的1.5小时（90分钟）、24小时、48小时和96小时。96小时后，存活率为100%。皮质醇和葡萄糖水平在0.5小时时较高，分别在48小时和24小时恢复到基线水平。乳酸脱氢酶水平在暴露于空气后1.5小时最高，24小时恢复到正常值。在血气值和电解质方面记录到了一些变化。关于血液学和血液化学，在整个96小时的实验过程中，暴露于空气对血细胞容积和天门冬氨酸氨基转移酶没有影响。碱性磷酸酶的值在0小时、1.5小时和24小时最高。总蛋白在0小时和1.5小时之间相似，在96小时最低，而谷丙转氨酶在0.5小时最高。白细胞在0.5小时、1.5小时、48小时和96小时最高，而红细胞在96小时最高。96小时后，亚历山大低冠鲶幼鱼能够重新建立应激的主要指标（皮质醇、葡萄糖和乳酸脱氢酶），而其他指标（血液学、生化和血气指标）则表现出代偿性变化以实现正常生理重建。
