Galbraith R M, Goust J M, Fudenberg H H
J Exp Med. 1977 Dec 1;146(6):1821-6. doi: 10.1084/jem.146.6.1821.
The presence of phytohemagglutinin or pokeweed mitogen in cultures of human peripheral blood mononuclear cells in agar is known to stimulate the formation of lymphoid colonies. We now report that similar colonies can be induced in the absence of plant lectins upon addition of filtered and ultracentrifuged conditioned medium (CM) obtained from certain human lymphoblastoid cell lines. Colony formation required at least 6 X 10(5) mononuclear cells per milliliter, and optimum results were obtained at concentrations of 1 X 10(6) cells/ml in the presence of 20% CM (50-500 colonies per 10(6) cells cultured). Individual cells within colonies displayed uniform morphological characteristics of lymphoid cells, and the majority formed rosettes with sheep erythrocytes, suggesting that they were of T-cell type.
已知在琼脂中培养的人外周血单个核细胞中存在植物血凝素或商陆有丝分裂原可刺激淋巴集落的形成。我们现在报告,在添加从某些人淋巴母细胞系获得的经过滤和超速离心的条件培养基(CM)后,在没有植物凝集素的情况下也可诱导形成类似的集落。集落形成每毫升至少需要6×10⁵个单个核细胞,在20%CM存在的情况下,细胞浓度为1×10⁶个/ml时可获得最佳结果(每培养10⁶个细胞有50 - 500个集落)。集落内的单个细胞表现出淋巴细胞的均匀形态特征,并且大多数与绵羊红细胞形成玫瑰花结,表明它们是T细胞类型。