Katsuoka Hiroyuki, Hamabe Naoya, Kato Chiemi, Hisamatsu Susumu, Baba Fujio, Taneishi Motohiro, Sasaki Toshiyuki, Ikegaya Atsushi, Inaba Zentaro
Izu Agricultural Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry, 3012 Inatori, Higashiizu, Kamo, Shizuoka 413-0411, Japan.
Department of Agricultural Production, Shizuoka Professional University Junior College of Agriculture, 678-1 Tomigaoka, Iwata, Shizuoka 438-8577, Japan.
Plant Biotechnol (Tokyo). 2022 Jun 25;39(2):93-100. doi: 10.5511/plantbiotechnology.21.1130a.
To introduce useful characteristics such as fragrance into (L.) and to expand the variation, we conducted crosses using as the seed parent and (L.) All. as the pollen parent. All the tested cross combinations between the three strains of and one strain of produced embryos, and healthy plants were obtained by ovule culture. The obtained plantlets had a white ray floret, and the leaf shape was intermediate to those of the parents. The individuals obtained from this cross were subjected to two methods to determine hybridity: flow cytometry analyses and cleaved amplified polymorphic sequence (CAPS) markers. For the CAPS marker, we selected the internal transcribed spacer (ITS) region, which is highly variable among the genera, as the region to be amplified. We selected restriction enzymes T120 I and II, which selectively cut common sequences in the genus , based on the sequence analysis of one parent strain each of and and alignment with known sequences of related species. Flow cytometry analyses and CAPS markers revealed that the individuals obtained from the cross between and are intergeneric hybrids. In addition, these established methods were capable of quickly and reliably identifying hybrids between and . This report shows for the first time that crossbreeding between (seed parent) and (pollen parent) is possible, and further development of breeding, such as the expansion of variation by intergeneric crosses, is expected.
为了将诸如香味等有用特性引入（某植物学名，此处原文未完整给出）并扩大其变异，我们以（某植物学名，此处原文未完整给出）作为母本，以（某植物学名，此处原文未完整给出）作为父本进行杂交。在（某植物学名，此处原文未完整给出）的三个菌株与（某植物学名，此处原文未完整给出）的一个菌株之间进行的所有测试杂交组合都产生了胚，并通过胚珠培养获得了健康植株。所获得的植株有白色的舌状花，叶形介于双亲之间。对由此杂交获得的个体采用两种方法来确定其杂种性：流式细胞术分析和酶切扩增多态性序列（CAPS）标记。对于CAPS标记，我们选择了在属间高度可变的内转录间隔区（ITS）作为要扩增的区域。基于（某植物学名，此处原文未完整给出）的一个亲本菌株各自的序列分析以及与相关物种已知序列的比对，我们选择了限制性内切酶T120 I和（另一酶名，此处原文未完整给出）II，它们能选择性地切割（某植物学名，此处原文未完整给出）属中的共同序列。流式细胞术分析和CAPS标记表明，从（某植物学名，此处原文未完整给出）与（某植物学名，此处原文未完整给出）杂交获得的个体是属间杂种。此外，这些既定方法能够快速且可靠地鉴定（某植物学名，此处原文未完整给出）与（某植物学名，此处原文未完整给出）之间的杂种。本报告首次表明（某植物学名，此处原文未完整给出）（母本）与（某植物学名，此处原文未完整给出）（父本）之间的杂交是可行的，并且预期（某植物学名，此处原文未完整给出）育种会有进一步发展，例如通过属间杂交扩大变异。
