Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education & Chemical Biology Key Laboratory of Hebei Province, College of Chemistry & and Environmental Science, Hebei University, Baoding, 071002, P. R. China.
CAS Key Laboratory of Magnetic Materials and Devices & Key Laboratory of Additive Manufacturing Materials of Zhejiang Province & Division of Functional Materials and Nanodevices, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo, 315201, P. R. China.
Adv Healthc Mater. 2018 Dec;7(24):e1801144. doi: 10.1002/adhm.201801144. Epub 2018 Oct 29.
Compared with other subtypes of breast cancer, triple-negative breast cancer (TNBC) is seriously threatening to human life. Therefore, it is a matter of urgency to develop multifunctional nanoprobes for visualized theranostics of TNBC, achieving specific targeting toward only TNBC, but not other subtypes. Nanoscale metal-organic frameworks (MOFs) show important potential in visualized theranostics of tumors, but it is critical to synthesize well-defined core-shell MOF-based nanocomposites by encapsulating a single nanoparticle within MOF. In this study, a TNBC-targeted peptide (ZD2)-engineered, and a single gold nanostar (AuNS) coated within MIL-101-NH (Fe) by coating MOF with four cycles, obtain well-defined core-shell AuNS@MOF-ZD2 nanocomposites, which are expected to achieve T -weighted magnetic resonance imaging and photothermal therapy (PTT) specifically targeting toward TNBC. The prepared AuNS@MOF-ZD2 nanocomposites possess good biocompatibility, efficient T -weighted magnetic resonance (MR) relaxivity and stable photothermal conversion ability with an efficiency of 40.5%. The in vitro and in vivo characterizations prove their performances of T -weighted MR and PTT with a low power density of 808 nm laser, achieving excellent theranostic efficacy in TNBC. Importantly, it is demonstrated that the prepared AuNS@MOF-ZD2 nanoprobes can specifically target TNBC cells (MDA-MB-231), but not other subtypes of breast cancer cells (MDA-MB-435, MDA-MB-468, and MCF-7), indicating their promising application in visualized theranostics of breast cancers with molecular classification.
与其他乳腺癌亚型相比，三阴性乳腺癌（TNBC）严重威胁着人类的生命。因此，开发用于 TNBC 可视化治疗的多功能纳米探针迫在眉睫，实现仅对 TNBC 的特异性靶向，而不是其他亚型。纳米尺度的金属-有机骨架（MOFs）在肿瘤的可视化治疗中显示出重要的潜力，但通过将单个纳米粒子封装在 MOF 中合成具有明确核壳结构的 MOF 基纳米复合材料是至关重要的。在这项研究中，通过用 MOF 包覆 4 个循环，得到了一种 TNBC 靶向肽（ZD2）工程化的、单个金纳米星（AuNS）包裹在 MIL-101-NH（Fe）内的核壳结构的 AuNS@MOF-ZD2 纳米复合材料，有望实现特异性针对 TNBC 的 T1 加权磁共振成像和光热治疗（PTT）。所制备的 AuNS@MOF-ZD2 纳米复合材料具有良好的生物相容性、高效的 T1 加权磁共振（MR）弛豫率和稳定的光热转换能力，效率为 40.5%。体外和体内特性证明了它们在 T1 加权 MR 和 PTT 方面的性能，在 808nm 激光的低功率密度下，实现了 TNBC 的优异治疗效果。重要的是，证明了所制备的 AuNS@MOF-ZD2 纳米探针可以特异性靶向 TNBC 细胞（MDA-MB-231），而不是其他乳腺癌细胞（MDA-MB-435、MDA-MB-468 和 MCF-7），表明它们在具有分子分类的乳腺癌可视化治疗中有很好的应用前景。
