Department of Biotechnology, Yonsei University, Seoul, Korea.
Juvic Inc, Seoul, Korea.
J Cosmet Dermatol. 2019 Aug;18(4):1083-1091. doi: 10.1111/jocd.12807. Epub 2018 Oct 29.
Dissolving microneedles (DMNs), microscale needles with a biodegradable polymer matrix, have been widely investigated for transdermal drug delivery. However, the restricted drug loading space of DMNs limited the delivery of the desired quantity of active compounds. In this study, we developed novel combinatorial therapies involving sequential application of adenosine-loaded DMN (Ad-DMN) patches and a topical adenosine-loaded cream (Ad-cream). The application of DMNs created skin channels, which delivered encapsulated drugs from both the DMNs and cream. The use of combinatorial therapies can maximize drug delivery.
To compare the efficacy of combinatorial therapies and Ad-cream application, a double-blind clinical test was conducted over 10 weeks on 21 females with wrinkles around their eyes, and the skin parameters such as wrinkles, dermal density, elasticity, and hydration were analyzed. The skin irritation test was assessed by expert interviewers to elucidate undesirable side effects.
The combinatorial therapies showed statistically significant efficacy for the improvement of average depth of wrinkles, dermal density, elasticity, and hydration after an 8-week application (P < 0.001). Adverse effects on the skin were not observed in any subject during the test period.
The efficacy and safety results showed that the combinatorial therapies were a safe and outstanding innovation for the optimization of transdermal therapy.
溶解微针（DMN）是一种具有可生物降解聚合物基质的微尺度针，已广泛用于透皮药物输送。然而，DMN 的受限药物装载空间限制了所需数量的活性化合物的输送。在这项研究中，我们开发了涉及顺序应用载有腺苷的 DMN（Ad-DMN）贴片和局部载有腺苷的乳膏（Ad-cream）的新型组合疗法。DMNs 的应用创建了皮肤通道，从 DMNs 和乳膏输送封装的药物。组合疗法的使用可以最大限度地提高药物输送。
为了比较组合疗法和 Ad-cream 应用的疗效，对 21 名眼部周围有皱纹的女性进行了为期 10 周的双盲临床试验，分析了皱纹、真皮密度、弹性和水分等皮肤参数。通过专家访谈评估皮肤刺激试验，以阐明不良副作用。
组合疗法在 8 周应用后显示出改善平均皱纹深度、真皮密度、弹性和水分的统计学显著疗效（P < 0.001）。在测试期间，任何受试者的皮肤均未观察到不良反应。
疗效和安全性结果表明，组合疗法是优化透皮治疗的安全且出色的创新。
