Veckenstedt A, Güttner J, Béládi I
Antiviral Res. 1987 Mar;7(3):169-78. doi: 10.1016/0166-3542(87)90005-2.
ABD2F1 mice were infected intraperitoneally (i.p.) or intranasally (i.n.) with Mengo or Sindbis virus and treated with either crude murine alpha/beta interferon (MuIFN-alpha/beta) or quercetin, or both. MuIFN-alpha/beta given i.p. or intramuscularly (i.m.) 1-3 h before the infection had a dose-dependent protective effect regardless of the route of administration. When given after the infection, IFN did not show any effect. Oral quercetin, capable of protecting cardio, i.e. Mengo virus-infected mice, failed to show antiviral efficacy in Sindbis virus-infected animals. Of various combinations of quercetin and MuIFN-alpha/beta, a certain well defined regimen resulted in a significant enhancement of protection in Mengo, but not Sindbis, virus-infected mice. A marginally effective treatment regimen of quercetin (20 mg/kg, given 12 h before Mengo virus infection, and 10 mg/kg given both 1 h before and 12 h after infection) potentiated the activity of a single dose of MuIFN-alpha/beta (5000 IU 3 h prior to infection), giving 85-100% survivors compared to 50% for MuIFN-alpha/beta when applied alone (p less than 0.001).
将Mengo病毒或辛德毕斯病毒经腹腔内（i.p.）或鼻内（i.n.）感染ABD2F1小鼠，并用粗制鼠α/β干扰素（MuIFN-α/β）或槲皮素或两者进行治疗。在感染前1 - 3小时经腹腔内或肌肉内（i.m.）给予MuIFN-α/β，无论给药途径如何，均具有剂量依赖性保护作用。在感染后给予干扰素则无任何效果。能够保护心脏的口服槲皮素，即对感染Mengo病毒的小鼠有效，但在感染辛德毕斯病毒的动物中未显示出抗病毒效果。在槲皮素和MuIFN-α/β的各种组合中，某种明确的方案在感染Mengo病毒而非辛德毕斯病毒的小鼠中显著增强了保护作用。一种槲皮素的轻度有效治疗方案（在感染Mengo病毒前12小时给予20mg/kg，在感染前1小时和感染后12小时均给予10mg/kg）增强了单剂量MuIFN-α/β（感染前3小时给予5000IU）的活性，单独应用MuIFN-α/β时存活率为50%，而联合应用时存活率为85 - 100%（p小于0.001）。
