Mabrouk Mostafa, Kumar Pradeep, Choonara Yahya E, du Toit Lisa C, Pillay Viness
Wits Advanced Drug Delivery Platform Research Unit, Department of Pharmacy and Pharmacology, School of Therapeutics, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, 7 York Road, Parktown 2109, South Africa.
Refractories, Ceramics and Building Materials Department, National Research Centre, 33 El Bohouthst. (former EL Tahrirst.), Dokki, Giza P.O.12622, Egypt.
Materials (Basel). 2018 Oct 29;11(11):2128. doi: 10.3390/ma11112128.
The present work aims to electrospin a triple layered wound patch for potential treatment of diabetic foot ulcers (DFU). The patch consisted of poly(acrylic acid) (PAA) as the skin contacting layer, polyvinyl pyrrolidone (PVP) as the middle layer, and polycaprolactone (PCL) as the outermost layer, wherein the PVP layer was loaded in situ with an antibiotic (ciprofloxacin, CFX). Morphology and mechanical properties were investigated using SEM and texture analysis. Patch quality was studied with regards to wettability, adherence, water resistance, and moisture uptake of individual layers. SEM results confirmed the fibrous and membranous nature of layers with a nano-to-micro size range. Mechanical properties of the composite patch demonstrated a tensile strength of 12.8 ± 0.5 MPa, deformation energy of 54.35 ± 0.1 J/m³, and resilience of 17.8 ± 0.7%, which were superior compared to individual layers. Patch quality tests revealed that the PCL layer showed very low wettability, adherence, and moisture uptake compared to the PVP and PAA layers. In vitro drug release data revealed an increase in cumulative drug release with higher drug loading. The results above confirm the potential of a triple layered, tripolymeric, wound patch for DFU intervention.
本研究旨在通过静电纺丝制备一种三层伤口贴片，用于潜在治疗糖尿病足溃疡（DFU）。该贴片由作为皮肤接触层的聚丙烯酸（PAA）、作为中间层的聚乙烯吡咯烷酮（PVP）和作为最外层的聚己内酯（PCL）组成，其中PVP层原位负载了一种抗生素（环丙沙星，CFX）。使用扫描电子显微镜（SEM）和质地分析研究了其形态和力学性能。从各层的润湿性、粘附性、耐水性和吸湿性方面研究了贴片质量。SEM结果证实了各层具有纳米到微米尺寸范围的纤维状和膜状性质。复合贴片的力学性能显示其拉伸强度为12.8±0.5MPa，变形能为54.35±0.1J/m³，回弹性为17.8±0.7%，与各单层相比更优越。贴片质量测试表明，与PVP层和PAA层相比，PCL层的润湿性、粘附性和吸湿性非常低。体外药物释放数据显示，随着药物负载量的增加，累积药物释放量增加。上述结果证实了三层三元伤口贴片用于DFU干预的潜力。
