Wang Linlin, Su Qiwen, Liu Yi, Yimamumaimaiti Tajiguli, Hu Dandan, Zhu Jun-Jie, Zhang Jian-Rong
Shaanxi Key Laboratory of Chemical Additives for Industry, College of Chemistry and Chemical Engineering, Shaanxi University of Science and Technology Xi'an 710021 China.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University Nanjing 210023 P. R. China
Chem Sci. 2022 Sep 30;13(41):12136-12143. doi: 10.1039/d2sc04242h. eCollection 2022 Oct 26.
Accelerating diabetes-related chronic wound healing is a long-sought-after goal in diabetes management. However, therapeutic strategies based on antibiotics or catalysts still face great challenges to break the limitations of antimicrobial resistance, low HO and the blocking effect of bacterial biofilms on antibiotic/catalyst penetration. Herein, we reported a glucose biofuel cell-powered and drug-free antibacterial patch, which consisted of an MAF-7 protected glucose oxidase/horseradish peroxidase anode and a horseradish peroxidase cathode, for treating diabetic wounds. This self-powered patch could take high blood glucose as fuel to generate electricity and abundant reactive oxygen species (ROS) , synergistically regulating local hyperglycemia and breaking the limitations of insufficient ROS caused by low HO levels. In particular, the electric field created by the GBFC could drive the negatively charged bacteria to adhere firmly to the electrode surface. As a result, the ROS produced on the electrodes was localized to the bacteria, realizing precise sterilization. experiments confirmed that this self-powered patch enabled the wounds on diabetic mice to take a mere 10 days to eliminate inflammation and form mature skin with new hair follicles, demonstrating its great potential in treating bacteria-infected diabetic wounds.
加速糖尿病相关慢性伤口愈合是糖尿病管理中长期追求的目标。然而，基于抗生素或催化剂的治疗策略在突破抗微生物耐药性、低过氧化氢水平以及细菌生物膜对抗生素/催化剂渗透的阻碍作用等方面仍面临巨大挑战。在此，我们报道了一种葡萄糖生物燃料电池供电的无药抗菌贴片，其由MAF-7保护的葡萄糖氧化酶/辣根过氧化物酶阳极和辣根过氧化物酶阴极组成，用于治疗糖尿病伤口。这种自供电贴片可以将高血糖作为燃料来发电并产生大量活性氧（ROS），协同调节局部高血糖并突破低过氧化氢水平导致的活性氧不足的限制。特别地，葡萄糖生物燃料电池产生的电场可以驱使带负电荷的细菌牢固地粘附在电极表面。结果，电极上产生的活性氧定位于细菌，实现精确杀菌。实验证实，这种自供电贴片能使糖尿病小鼠的伤口仅需10天就能消除炎症并形成带有新毛囊的成熟皮肤，证明其在治疗细菌感染的糖尿病伤口方面具有巨大潜力。
