Department of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, UK.
Drug Deliv Transl Res. 2013 Dec;3(6):531-41. doi: 10.1007/s13346-013-0164-9.
We report the controlled release of the antibiotic tetracycline (tet) HCl from a triple-layered electrospun matrix consisting of a central layer of poly(ethylene-co-vinyl acetate (PEVA) sandwiched between outer layers of poly-ε-caprolactone (PCL). These micro/nanofibre layers with tet successfully encapsulated (essentially quantitatively at 3 and 5 % w/w) in each layer, efficiently inhibited the growth of a panel of bacteria, including clinical isolates, as shown by a modified Kirby-Bauer disc assay. Furthermore, they demonstrated high biological activity in increasingly complex models of biofilm formation (models that are moving closer to the situation in a wound) by stopping biofilm formation, by killing preformed biofilms and killing mature, dense biofilm colonies of Staphylococcus aureus MRSA252. Tet is clinically useful with potential applications in wound healing and especially in complicated skin and skin-structure infections; electrospinning provides good encapsulation efficiency of tet within PCL/PEVA/PCL polymers in micro/nanofibre layers which display sustained antibiotic release in formulations that are anti-biofilm.
我们报告了一种由三层电纺基质组成的抗生素四环素（tet）HCl 的控制释放，该基质由聚（乙烯-共-醋酸乙烯酯（PEVA）的中央层夹在聚己内酯（PCL）的外层之间。这些带有 tet 的微/纳米纤维层成功地封装在每一层中（实质上在 3%和 5%w/w 时定量封装），有效地抑制了一系列细菌的生长，包括临床分离株，这通过改良 Kirby-Bauer 圆盘试验证明。此外，它们通过阻止生物膜形成、杀死已形成的生物膜和杀死成熟、密集的金黄色葡萄球菌 MRSA252 生物膜菌落，在越来越复杂的生物膜形成模型中表现出高生物活性（这些模型更接近伤口中的情况）。四环素在临床上具有应用潜力，可用于伤口愈合，尤其是在复杂的皮肤和皮肤结构感染中；电纺技术为 PCL/PEVA/PCL 聚合物中的 tet 提供了良好的封装效率，在抗生物膜制剂中显示出持续的抗生素释放。
