用于检测天然和实验性鼠巨细胞病毒抗体的血清学检测方法比较
Comparison of serological tests for the detection of antibody to natural and experimental murine cytomegalovirus.
作者信息
Lussier G, Guénette D, Descôteaux J P
出版信息
Can J Vet Res. 1987 Apr;51(2):249-52.
Three serological tests, i.e. complement fixation test, indirect immunofluorescent assay, and enzyme-linked immunosorbent assay (ELISA) were compared for sensitivity in the detection and titration of murine cytomegalovirus antibody. The three tests were compared using sera from experimentally inoculated and naturally infected mice bled at intervals from 3 to 140 days postinfection. In the acute infection, complement fixation and indirect immunofluorescent assay tests were of comparable sensitivity for early detection of antibody, whereas the ELISA was less sensitive. In persistent infection, higher titers were recorded with ELISA. Since murine cytomegalovirus has been shown to exert significant effects on the immune response of infected mice, this antigen should be included routinely in viral antibody screening programs.
比较了三种血清学检测方法，即补体结合试验、间接免疫荧光测定法和酶联免疫吸附测定法（ELISA）在检测和滴定小鼠巨细胞病毒抗体方面的敏感性。使用实验接种和自然感染小鼠在感染后3至140天期间间隔采集的血清对这三种检测方法进行了比较。在急性感染中，补体结合试验和间接免疫荧光测定法在早期检测抗体方面具有相当的敏感性，而ELISA则较不敏感。在持续性感染中，ELISA记录到更高的滴度。由于已证明小鼠巨细胞病毒对感染小鼠的免疫反应有显著影响，该抗原应常规纳入病毒抗体筛查程序。
Zotero 插件