Turk J Med Sci. 2018 Oct 31;48(5):985-992. doi: 10.3906/sag-1803-135.
Background/aim: Thiol-disulfide homeostasis is an important antioxidant defense mechanism. This study was conducted to investigate dynamic thiol-disulfide homeostasis in patients with hepatitis B virus-related chronic hepatitis and liver cirrhosis. Materials and methods: Seventy-one treatment-naive patients with chronic hepatitis B (CHB), 50 patients with hepatitis B virusassociated liver cirrhosis, and 45 healthy controls were included in the study. Serum total and native thiol concentrations and serum disulfide concentrations were measured using an automated method. Results: Mean serum total thiol concentrations in the control, CHB, and cirrhosis groups were 481.64 ± 37.87 µmol/L, 438.50 ± 71.35 µmol/L, and 358.07 ± 80.47 µmol/L, respectively (P < 0.001), and mean serum native thiol concentrations in the control, CHB, and cirrhosis groups were 452.92 ± 36.43 µmol/L, 400.16 ± 65.92 µmol/L, and 328.15 ± 74.91 µmol/L, respectively (P < 0.001). Mean serum disulfide concentrations in the control, CHB, and cirrhosis groups were 14.38 ± 3.38 µmol/L, 19.19 ± 6.16 µmol/L, and 14.98 ± 5.53 µmol/L, respectively (P < 0.001). There was a progressive decrease in both mean serum native and total thiol concentrations parallel to the liver fibrosis stage. Conclusion: : Thiol-disulfide homeostasis is disturbed in patients with hepatitis B virus-related chronic hepatitis and liver cirrhosis.
背景/目的：硫醇-二硫键动态平衡是一种重要的抗氧化防御机制。本研究旨在探讨乙型肝炎病毒相关慢性肝炎和肝硬化患者硫醇-二硫键动态平衡的变化。
本研究纳入了 71 例未经治疗的慢性乙型肝炎（CHB）患者、50 例乙型肝炎病毒相关肝硬化患者和 45 例健康对照者。采用自动化方法测定血清总巯基和天然巯基浓度及血清二硫键浓度。
对照组、CHB 组和肝硬化组的血清总巯基浓度均值分别为 481.64 ± 37.87 µmol/L、438.50 ± 71.35 µmol/L 和 358.07 ± 80.47 µmol/L（P < 0.001），血清天然巯基浓度均值分别为 452.92 ± 36.43 µmol/L、400.16 ± 65.92 µmol/L 和 328.15 ± 74.91 µmol/L（P < 0.001）。对照组、CHB 组和肝硬化组的血清二硫键浓度均值分别为 14.38 ± 3.38 µmol/L、19.19 ± 6.16 µmol/L 和 14.98 ± 5.53 µmol/L（P < 0.001）。随着肝纤维化程度的加重，血清天然巯基和总巯基浓度逐渐降低。
乙型肝炎病毒相关慢性肝炎和肝硬化患者的硫醇-二硫键动态平衡受到干扰。
