School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287, USA.
Center for Molecular Design and Biomimetics, Biodesign Institute, Arizona State University, Tempe, AZ, 85287, USA.
Nat Commun. 2018 Nov 2;9(1):4579. doi: 10.1038/s41467-018-07039-7.
Molecular knots represent one of the most extraordinary topological structures in biological polymers. Creating highly knotted nanostructures with well-defined and sophisticated geometries and topologies remains challenging. Here, we demonstrate a general strategy to design and construct highly knotted nucleic acid nanostructures, each weaved from a single-stranded DNA or RNA chain by hierarchical folding in a prescribed order. Sets of DNA and RNA knots of two- or three-dimensional shapes have been designed and constructed (ranging from 1700 to 7500 nucleotides), and they exhibit complex topological features, with high crossing numbers (from 9 up to 57). These single-stranded DNA/RNA knots can be replicated and amplified enzymatically in vitro and in vivo. This work establishes a general platform for constructing nucleic acid nanostructures with complex molecular topologies.
分子纽结是生物聚合物中最非凡的拓扑结构之一。用具有明确定义和复杂几何形状和拓扑结构的高度纽结纳米结构仍然具有挑战性。在这里，我们展示了一种设计和构建高度纽结核酸纳米结构的通用策略，这些纳米结构由单链 DNA 或 RNA 链通过按规定顺序分层折叠而成。已经设计和构建了具有二维或三维形状的 DNA 和 RNA 纽结集（从 1700 到 7500 个核苷酸），它们表现出复杂的拓扑特征，具有高交叉数（从 9 到 57）。这些单链 DNA/RNA 纽结可以在体外和体内通过酶复制和扩增。这项工作为构建具有复杂分子拓扑结构的核酸纳米结构建立了一个通用平台。
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