Stewart Jaimie Marie, Subramanian Hari K K, Franco Elisa
Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA, USA.
ChromaCode, Inc., Carlsbad, CA, USA.
Methods Mol Biol. 2022;2433:293-302. doi: 10.1007/978-1-0716-1998-8_18.
Artificial self-assembling RNA scaffolds can be produced from many types of RNA motifs that are rationally designed. These scaffolds are of interest as nanoscale organizers, with applications in drug delivery and synthetic cells. Here we describe design strategies, production methods, and imaging of micrometer-sized RNA nanotubes and lattices that assemble from RNA tiles comprising multiple distinct strands.
人工自组装RNA支架可由多种经过合理设计的RNA基序产生。这些支架作为纳米级组织者备受关注，可应用于药物递送和合成细胞。在这里，我们描述了由包含多个不同链的RNA片组装而成的微米级RNA纳米管和晶格的设计策略、生产方法及成像。
