梅毒螺旋体感染兔的体外细胞反应。III. 淋巴细胞促有丝分裂因子产生的损害。
In vitro cell response of Treponema pallidum-infected rabbits. III. Impairment in production of lymphocyte mitogenic factor.
作者信息
Wicher V, Wicher K
出版信息
Clin Exp Immunol. 1977 Sep;29(3):496-500.
Production of mitogenic factor was examined in rabbits infected intratesticularly with T. pallidum and in control animals injected with saline or saline extract of normal rabbits' testes. Lymph nodes and spleen from animals killed 2, 6 and 12 weeks after injection were used as the source of lymphocytes, cultured in serum-free medium in the presence of Reiter antigen. The active supernatants of lymph node cells (LNAS) and spleen cells (SPAS) were examined for the presence of mitogenic factor using normal rabbit peripheral lymphocytes. The LNAS of control animals showed a mitogenic index (MI) between 4 and 6 and the infected animals less than 2. The SPAS of infected and control rabbits showed an MI of less than 2. The lower mitogenicity in LNAS of infected and that of SPAS of infected and control animals seems to be due to the presence of inhibitors of DNA synthesis.
研究了经睾丸内接种梅毒螺旋体的家兔以及注射生理盐水或正常家兔睾丸生理盐水提取物的对照动物中促有丝分裂因子的产生情况。在注射后2周、6周和12周处死动物，取其淋巴结和脾脏作为淋巴细胞来源，在无血清培养基中于赖特抗原存在的情况下进行培养。使用正常家兔外周淋巴细胞检测淋巴结细胞活性上清液（LNAS）和脾细胞活性上清液（SPAS）中促有丝分裂因子的存在情况。对照动物的LNAS促有丝分裂指数（MI）在4至6之间，而感染动物的MI小于2。感染家兔和对照家兔的SPAS的MI均小于2。感染动物LNAS以及感染和对照动物SPAS中较低的促有丝分裂活性似乎是由于存在DNA合成抑制剂。
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