Department of Nutrition and Food Science, Princess Nora Bint Abdulrahman University, Kingdom of Saudi Arabia, Division of Nutritional Sciences, School of Biosciences, The University of Nottingham, United Kingdom.
Division of Nutritional Sciences, School of Biosciences, The University of Nottingham, United Kingdom.
Magnes Res. 2018 May 1;31(2):39-48. doi: 10.1684/mrh.2018.0436.
Given a possible anti-inflammatory role of magnesium in endothelial cells, the aim of this study was to investigate the effects of magnesium on human umbilical vein endothelial cell (HUVEC) viability, gene expression, and the pro-inflammatory response caused by a bacterial endotoxin (LPS). HUVECs were cultured at three different concentrations of magnesium sulphate (0.1 mM; control-1 mM; 5 mM) for 72 hours. Exposing the cells to LPS reduced cell viability in culture with low magnesium, but high magnesium protected the HUVECs from LPS-induced cell death. LPS-treated HUVECs cultured in low magnesium showed up-regulation of mRNA expression for pro-inflammatory factors and the expression of cytokine proteins, including IL-2, IL-3, IL-8, IL-15 and MCP-1. This was associated with greater adhesion of monocytes to the cells. In contrast, high magnesium decreased the expression of inflammatory factors and cytokines. The study found that LPS activation of the expression of many pro-inflammatory factors is exacerbated in the presence of low magnesium concentration whilst a high magnesium concentration partly inhibited the inflammatory response to LPS.
鉴于镁在血管内皮细胞中可能具有抗炎作用，本研究旨在探讨镁对人脐静脉内皮细胞（HUVEC）活力、基因表达以及细菌内毒素（LPS）引起的促炎反应的影响。将 HUVEC 在三种不同浓度的硫酸镁（0.1 mM；对照-1 mM；5 mM）中培养 72 小时。用低镁孵育暴露于 LPS 的细胞会降低细胞活力，但高镁可保护 HUVEC 免受 LPS 诱导的细胞死亡。在低镁培养的 LPS 处理的 HUVEC 中，促炎因子的 mRNA 表达和细胞因子蛋白（包括 IL-2、IL-3、IL-8、IL-15 和 MCP-1）的表达上调。这与单核细胞与细胞的粘附增加有关。相比之下，高镁减少了炎症因子和细胞因子的表达。该研究发现，LPS 激活许多促炎因子的表达在低镁浓度存在下加剧，而高镁浓度部分抑制了 LPS 引起的炎症反应。
