College of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Sci Data. 2018 Nov 6;5:180222. doi: 10.1038/sdata.2018.222.
Because of poor differentiation among the members of genus Comamonas using phenotypic methods, human infections caused by C. kerstersii are sporadically reported in the literature. Here, we represent the first complete genome sequence of C. kerstersii 8943, which caused peritonitis in a patient with continuous ambulatory peritoneal dialysis (CAPD). The complete genome with no gaps was obtained using third-generation Pacific Biosciences (PacBio) RSII sequencing system with single-molecule real-time (SMRT) analysis. Protein-coding genes, rRNAs and tRNAs were predicted. Functional annotations of the genome using different databases revealed several genes related to pathogenicity including antibiotic resistance genes and prophages. Our work demonstrates that whole genome sequencing can enhance the resolution of clinical investigations and our data can be used as a reference genome during the rapid diagnosis of C. kerstersii infections in the future.
由于表型方法在鉴别苍白杆菌属成员方面的不足，文献中偶有报道由科尔氏苍白杆菌引起的人类感染。在这里，我们展示了导致持续性非卧床腹膜透析（CAPD）患者腹膜炎的科尔氏苍白杆菌 8943 的首个完整基因组序列。使用第三代太平洋生物科学（PacBio）RSII 测序系统和单分子实时（SMRT）分析获得了无间隙的完整基因组。预测了蛋白编码基因、rRNA 和 tRNA。使用不同数据库对基因组进行功能注释，揭示了一些与致病性相关的基因，包括抗生素抗性基因和原噬菌体。我们的工作表明，全基因组测序可以提高临床研究的分辨率，我们的数据可以在未来快速诊断科尔氏苍白杆菌感染时作为参考基因组使用。
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