Key Laboratory of Plant Resource Conservation and Sustainable Utilization/Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
University of Chinese Academy of Sciences, Beijing 100039, China.
Sci Data. 2018 Jan 16;5:170205. doi: 10.1038/sdata.2017.205.
Vibrio qinghaiensis sp.-Q67 (Vqin-Q67) is a freshwater luminescent bacterium that continuously emits blue-green light (485 nm). The bacterium has been widely used for detecting toxic contaminants. Here, we report the complete genome sequence of Vqin-Q67, obtained using third-generation PacBio sequencing technology. Continuous long reads were attained from three PacBio sequencing runs and reads >500 bp with a quality value of >0.75 were merged together into a single dataset. This resultant highly-contiguous de novo assembly has no genome gaps, and comprises two chromosomes with substantial genetic information, including protein-coding genes, non-coding RNA, transposon and gene islands. Our dataset can be useful as a comparative genome for evolution and speciation studies, as well as for the analysis of protein-coding gene families, the pathogenicity of different Vibrio species in fish, the evolution of non-coding RNA and transposon, and the regulation of gene expression in relation to the bioluminescence of Vqin-Q67.
青海弧菌 Q67（Vibrio qinghaiensis sp.-Q67，简称 Vqin-Q67）是一种淡水发光细菌，能持续发出蓝绿光（485nm）。该细菌已被广泛用于检测有毒污染物。在此，我们报告了 Vqin-Q67 的全基因组序列，该序列是使用第三代 PacBio 测序技术获得的。我们从三个 PacBio 测序运行中获得了连续的长读段，然后将质量值 >0.75 且长度 >500bp 的读段合并成一个单一数据集。这个高连续性的从头组装基因组没有基因组缺口，由两个染色体组成，包含大量的遗传信息，包括蛋白质编码基因、非编码 RNA、转座子和基因岛。我们的数据集可作为进化和物种形成研究的比较基因组，也可用于分析不同鱼类中弧菌属的致病性、非编码 RNA 和转座子的进化、与 Vqin-Q67 生物发光相关的基因表达调控，以及蛋白质编码基因家族的分析。
