Laboratorio de Biotecnología Molecular de Plantas, División de Biología Molecular, San Luis Potosí, Mexico.
CONACYT-Consorcio de Investigación, Inovación y Desarrollo para las Zonas Aridas (CIIDZA), Instituto Potosino de Investigación Científica y Tecnológica A. C., San Luis Potosí, S.L.P., Mexico.
Sci Rep. 2018 Nov 6;8(1):16427. doi: 10.1038/s41598-018-34500-w.
Trichoderma spp colonizes the plant rhizosphere and provides pathogen resistance, abiotic stress tolerance, and enhance growth and development. We evaluated the Arabidopsis-Trichoderma interaction using a split system in which Trichoderma atroviride and Trichoderma virens were grown on PDA or MS medium. Arabidopsis growth was significantly increased at 3 and 5 days post-inoculation with both Trichoderma species, when the fungal strains were grown on PDA in split interaction. The analysis of DR5:uidA reporter line revealed a greater auxin accumulation in root tips when the fungi were grown on PDA in a split interaction. The root hair-defective phenotype of Arabidopsis rhd6 mutant was reverted with both Trichoderma species, even in split interactions. At 12 °C, Trichoderma species in split interactions were able to mitigate the effects of cold stress on the plant, and also Trichoderma induced the AtERD14 expression, a cold related gene. Volatile organic compounds analysis revealed that Trichoderma strains produce mainly sesquiterpenes, and that the type and abundance of these compounds was dependent on the fungal strain and the culture medium. Our results show that fungal nutrition is an important factor in plant growth in a split interaction.
木霉属（Trichoderma spp.）定殖于植物根际，提供对病原体的抗性、非生物胁迫耐受性，并促进生长和发育。我们使用分裂系统评估了拟南芥与木霉属的相互作用，其中深绿木霉（Trichoderma atroviride）和绿木霉（Trichoderma virens）在 PDA 或 MS 培养基上生长。当真菌在分裂相互作用中在 PDA 上生长时，拟南芥在接种后 3 天和 5 天的生长显著增加。对 DR5:uidA 报告系的分析表明，当真菌在分裂相互作用中在 PDA 上生长时，根尖处积累了更多的生长素。木霉属在分裂相互作用中可以逆转拟南芥 rhd6 突变体的根毛缺陷表型，即使在分裂相互作用中也是如此。在 12°C 下，分裂相互作用中的木霉属能够减轻植物的冷胁迫影响，并且木霉属诱导了冷相关基因 AtERD14 的表达。挥发性有机化合物分析表明，木霉属菌株主要产生倍半萜，这些化合物的类型和丰度取决于真菌菌株和培养基。我们的结果表明，真菌营养是分裂相互作用中植物生长的一个重要因素。
