Greenberg Samuel R, Tan Weimin, Lee Wei-Lih
Department of Biological Sciences, Dartmouth College, Hanover, NH, 03755, USA.
Biophys Rev. 2018 Dec;10(6):1631-1636. doi: 10.1007/s12551-018-0472-x. Epub 2018 Nov 6.
In both animals and fungi, spindle positioning is dependent upon pulling forces generated by cortically anchored dynein. In animals, cortical anchoring is accomplished by a ternary complex containing the dynein-binding protein NuMA and its cortical attachment machinery. The same function is accomplished by Num1 in budding yeast. While not homologous in primary sequence, NuMA and Num1 appear to share striking similarities in their mechanism of function. Here, we discuss evidence supporting that Num1 in fungi is a functional homolog of NuMA due to their similarity in domain organization and role in the generation of cortical pulling forces.
在动物和真菌中，纺锤体定位均依赖于皮层锚定的动力蛋白产生的拉力。在动物中，皮层锚定是由一个包含动力蛋白结合蛋白NuMA及其皮层附着机制的三元复合物完成的。在芽殖酵母中，Num1发挥相同的功能。虽然NuMA和Num1在一级序列上不具有同源性，但它们在功能机制上似乎有显著的相似之处。在这里，我们讨论了相关证据，这些证据支持真菌中的Num1是NuMA的功能同源物，因为它们在结构域组织以及在产生皮层拉力方面的作用具有相似性。
