CCDC102B 参与中心体连接体的组装和中心体黏合。
CCDC102B functions in centrosome linker assembly and centrosome cohesion.
机构信息
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China.
Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China
出版信息
J Cell Sci. 2018 Dec 3;131(23):jcs222901. doi: 10.1242/jcs.222901.
The proteinaceous centrosome linker is an important structure that allows the centrosome to function as a single microtubule-organizing center (MTOC) in interphase cells. However, the assembly mechanism of the centrosome linker components remains largely unknown. In this study, we identify CCDC102B as a new centrosome linker protein that is required for maintaining centrosome cohesion. CCDC102B is recruited to the centrosome by C-Nap1 (also known as CEP250) and interacts with the centrosome linker components rootletin and LRRC45. CCDC102B decorates and facilitates the formation of rootletin filaments. Furthermore, CCDC102B is phosphorylated by Nek2A (an isoform encoded by ) and is disassociated from the centrosome at the onset of mitosis. Together, our findings reveal a molecular role for CCDC102B in centrosome cohesion and centrosome linker assembly.This article has an associated First Person interview with the first authors of the paper.
蛋白质中心体连接蛋白是一种重要的结构，使中心体在间期中作为单个微管组织中心（MTOC）发挥作用。然而，中心体连接蛋白成分的组装机制在很大程度上仍然未知。在这项研究中，我们鉴定出 CCDC102B 是一种新的中心体连接蛋白，它对于维持中心体的凝聚力是必需的。CCDC102B 通过 C-Nap1（也称为 CEP250）被招募到中心体，并与中心体连接蛋白成分根蛋白和 LRRC45 相互作用。CCDC102B 对根蛋白丝进行装饰并促进其形成。此外，CCDC102B 被 Nek2A（一种由 编码的同工型）磷酸化，并在有丝分裂开始时从中心体解离。总之，我们的发现揭示了 CCDC102B 在中心体凝聚和中心体连接蛋白组装中的分子作用。本文有该论文第一作者的相关第一人称采访。
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