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种植体表面的牙髓干细胞：一项体外研究。

Dental Pulp Stem Cells on Implant Surface: An In Vitro Study.

机构信息

Multidisciplinary Departments of Medical-Surgical and Dental Specialties, Second University of Naples, Naples 80100, Italy.

Department of Biomedical and Dental Sciences and Morphological and Functional Imaging, Messina University, Messina 98100, Italy.

出版信息

Biomed Res Int. 2021 Mar 23;2021:3582342. doi: 10.1155/2021/3582342. eCollection 2021.

DOI:10.1155/2021/3582342
PMID:33834063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8012148/
Abstract

In the field of biology and medicine, one hears often about stem cells and their potential. The dental implant new surfaces, subjected to specific treatments, perform better and allow for quicker healing times and better clinical performance. The purpose of this study is to evaluate from a biological point of view the interaction and cytotoxicity between stem cells derived from dental pulp (DPSCs) and titanium surfaces. Through the creation of complex cells/implant, this study is aimed at analyzing the cytotoxicity of dental implant surfaces (Myth (Maipek Manufacturer Industrial Care, Naples, Italy)) and the adhesion capacity of cells on them and at considering the essential factors for implant healing such as osteoinduction and vasculogenesis. These parameters are pointed out through histology (3D cell culture), immunofluorescence, proliferation assays, scanning electron microscopy, and PCR investigations. The results of the dental implant surface and its interaction with the DPSCs are encouraging, obtaining results increasing the mineralization of the tissues. The knowledge of this type of interaction, highlighting its chemical and biological features, is certainly also an excellent starting point for the development of even more performing surfaces for having better healing in the oral surgical procedures related to dental implant positioning.

摘要

在生物学和医学领域，人们经常听到关于干细胞及其潜力的信息。经过特定处理的牙科种植体新表面表现更好，允许更快的愈合时间和更好的临床效果。本研究的目的是从生物学角度评估牙髓干细胞（DPSCs）和钛表面之间的相互作用和细胞毒性。通过创建复杂的细胞/植入物，本研究旨在分析牙科植入物表面（Myth（Maipek 制造商工业护理，那不勒斯，意大利））的细胞毒性和细胞在其上的粘附能力，并考虑到植入物愈合的重要因素，如成骨诱导和血管生成。这些参数通过组织学（3D 细胞培养）、免疫荧光、增殖测定、扫描电子显微镜和 PCR 研究进行指出。牙科植入物表面及其与 DPSCs 的相互作用的结果令人鼓舞，获得了增加组织矿化的结果。了解这种相互作用，突出其化学和生物学特性，无疑也是开发更具性能的表面的良好起点，以便在与牙科植入物定位相关的口腔外科手术中获得更好的愈合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cd9/8012148/5c9894ef3a5f/BMRI2021-3582342.009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cd9/8012148/5c9894ef3a5f/BMRI2021-3582342.009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cd9/8012148/f4effadb8608/BMRI2021-3582342.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cd9/8012148/ee0ae3643240/BMRI2021-3582342.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cd9/8012148/5f21f57cfc9c/BMRI2021-3582342.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3cd9/8012148/5c9894ef3a5f/BMRI2021-3582342.009.jpg

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