在两份人类肝细胞癌的DNA样本中鉴定出转化基因hst。
Identification of transforming genes as hst in DNA samples from two human hepatocellular carcinomas.
作者信息
Nakagama H, Ohnishi S, Imawari M, Hirai H, Takaku F, Sakamoto H, Terada M, Nagao M, Sugimura T
出版信息
Jpn J Cancer Res. 1987 Jul;78(7):651-4.
In a DNA-mediated transfection assay using NIH3T3 cells, the DNAs from two out of twelve human hepatocellular carcinomas gave transformants. The transforming gene was identified as hst, which was originally found in DNAs from stomach cancers and a noncancerous portion of stomach mucosa, the putative product being a growth factor. Transcripts were not detected in the original HCC 1 and HCC2 upon Northern blot analysis. The possible mechanisms involved in the hst gene activation are discussed.
在一项使用NIH3T3细胞的DNA介导转染试验中，12例人类肝细胞癌中有2例的DNA产生了转化细胞。转化基因被鉴定为hst，该基因最初在胃癌和胃黏膜非癌部分的DNA中发现，推测其产物为一种生长因子。Northern印迹分析显示，在原始的HCC 1和HCC2中未检测到转录本。文中讨论了hst基因激活可能涉及的机制。
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