Smialek M, Kaczorek E, Szczucińska E, Burchardt S, Kowalczyk J, Tykałowski B, Koncicki A
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland.
Department of Microbiology and Clinical Immunology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland.
Pol J Vet Sci. 2019 Mar;22(1):5-10. doi: 10.24425/pjvs.2018.125616.
The number of human cases of salmonellosis in the EU was 94,625 in 2015. Considering the source of these infections, Salmonella spp. was most frequently detected in broiler chicken meat and Salmonella Enteritidis (SE) was the most commonly reported serovar. The efficacy of probiotics in limiting Salmonella spp. infection in poultry has been demonstrated in numerous papers. The administration of probiotics at the level of primary production reduces the risk of contamination of poultry food products with Salmonella spp. A study was carried out in order to determine the potential for reducing the Salmonella spp. population in broiler chickens with the use of the Lavipan (JHJ, Poland) probiotic that comprised selected stains of lactic acid bacteria and Saccharomyces cervisae. Salmonella spp.-free broiler chickens were divided into two groups and received the same feed with (group L) or without (group C) the probiotic throughout the experiment. All day-old chickens were infected per os with SE. Samples of cecum content were collected 2, 4, and 6 weeks after SE infection and pectoral muscles were collected 6 weeks following SE infection for the evaluation of the SE population number. Serum samples for serological examinations were collected 6 weeks after infection. Six weeks after infection, the number of SE-positive cecal samples was lower in the L group (12.5% positive) in comparison to the C group (87.5%). Similar results were demonstrated for the muscle samples (25% in contrast to 87.5%). At the same time, in both cases, the SE CFU/g was significantly lower in the L group. The results of our study indicate that Lavipan was capable of reducing the population of SE in the gastrointestinal tract, which eventually improved the hygienic parameters of the pectoral muscles. Four weeks after infection, SE was not detected in any of the experimental groups. In both groups, no specific anti-SE antibodies were detected.
2015年欧盟沙门氏菌病的人类病例数为94,625例。考虑到这些感染的来源，沙门氏菌属最常于肉鸡中检测到，肠炎沙门氏菌（SE）是最常报告的血清型。众多论文已证明益生菌在限制家禽沙门氏菌属感染方面的功效。在初级生产水平施用益生菌可降低家禽食品被沙门氏菌属污染的风险。开展了一项研究，以确定使用包含选定乳酸菌菌株和酿酒酵母的Lavipan（波兰JHJ公司）益生菌减少肉鸡体内沙门氏菌属数量的潜力。无沙门氏菌属的肉鸡被分为两组，在整个实验过程中，一组（L组）接受添加益生菌的相同饲料，另一组（C组）接受不添加益生菌的相同饲料。所有一日龄雏鸡经口感染SE。在SE感染后2周、4周和6周收集盲肠内容物样本，并在SE感染后6周收集胸肌样本，以评估SE数量。感染后6周收集血清样本进行血清学检查。感染6周后，L组SE阳性盲肠样本数量（阳性率12.5%）低于C组（87.5%）。肌肉样本也得到了类似结果（分别为25%和87.5%）。同时，在这两种情况下，L组的SE CFU/g均显著更低。我们的研究结果表明，Lavipan能够减少胃肠道中的SE数量，最终改善胸肌的卫生参数。感染4周后，任何实验组均未检测到SE。两组均未检测到特异性抗SE抗体。
