Wu Shu, Zhang Qianyun, Cong Guanglei, Xiao Yunqi, Shen Yiru, Zhang Shan, Zhao Wenchang, Shi Shourong
Department of Feed and Nutrition, Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou, China.
Anim Nutr. 2023 Jun 8;14:450-460. doi: 10.1016/j.aninu.2023.06.001. eCollection 2023 Sep.
As a foodborne pathogen of global importance, serovar Enteritidis ( Enteritidis) is a threat to public health that is mainly spread by poultry products. Intestinal Enterobacteriaceae can inhibit the colonization of Enteritidis and are regarded as a potential antibiotic substitute. We investigated, in chicks, the anti- Enteritidis effects of () Nissle 1917, the most well-known probiotic member of Enterobacteriaceae. Eighty 1-d-old healthy female AA broilers were randomly divided into 4 groups, with 20 in each group, namely the negative control (group P), the Nissle 1917-treated group (group N), the . Enteritidis-infected group (group S) and the Nissle 1917-treated and . Enteritidis-infected group (group NS). From d 5 to 7, chicks in groups N and NS were orally gavaged once a day with Nissle 1917 and in groups P and S were administered the same volume of sterile PBS. At d 8, the chicks in groups S and NS were orally gavaged with . Enteritidis and in groups P and N were administered the same volume of sterile PBS. Sampling was conducted 24 h after challenge. Results showed that gavage of Nissle 1917 reduced the spleen index, loads, and inflammation ( < 0.05). It improved intestinal morphology and intestinal barrier function ( < 0.05). Enteritidis infection significantly reduced mRNA expression of angiotensin-converting enzyme 2 () and solute carrier family 6-member 19 () in the cecum and the content of Gly, Ser, Gln, and Trp in the serum ( < 0.05). Pretreatment with Nissle 1917 yielded mRNA expression of and in the cecum and levels of Gly, Ser, Gln, and Trp in the serum similar to that of uninfected chicks ( < 0.05). Additionally, Nissle 1917 altered cecum microbiota composition and enriched the abundance of , Lactobacillales, and Lachnospiraceae. These findings reveal that the probiotic Nissle 1917 reduced Enteritidis infection and shows enormous potential as an alternative to antibiotics.
作为一种具有全球重要性的食源性病原体，肠炎血清型（肠炎杆菌）是对公共卫生的一种威胁，主要通过家禽产品传播。肠道肠杆菌科细菌可抑制肠炎杆菌的定植，被视为一种潜在的抗生素替代品。我们在雏鸡中研究了肠杆菌科最著名的益生菌成员——（）Nissle 1917对肠炎杆菌的抗菌作用。80只1日龄健康雌性AA肉鸡随机分为4组，每组20只，即阴性对照组（P组）、Nissle 1917处理组（N组）、肠炎杆菌感染组（S组）和Nissle 1917处理且肠炎杆菌感染组（NS组）。从第5天到第7天，N组和NS组的雏鸡每天口服一次Nissle 1917，P组和S组给予相同体积的无菌PBS。在第8天，S组和NS组的雏鸡口服肠炎杆菌，P组和N组给予相同体积的无菌PBS。攻毒后24小时进行采样。结果表明，灌喂Nissle 1917可降低脾脏指数、负荷和炎症（<0.05）。它改善了肠道形态和肠道屏障功能（<0.05）。肠炎杆菌感染显著降低了盲肠中血管紧张素转换酶2（）和溶质载体家族6成员19（）的mRNA表达以及血清中甘氨酸、丝氨酸、谷氨酰胺和色氨酸的含量（<0.05）。用Nissle 1917预处理使盲肠中及的mRNA表达以及血清中甘氨酸、丝氨酸、谷氨酰胺和色氨酸的水平与未感染雏鸡相似（<0.05）。此外，Nissle 1917改变了盲肠微生物群组成，增加了、乳杆菌目和毛螺菌科的丰度。这些发现表明，益生菌Nissle 1917减少了肠炎杆菌感染，作为抗生素替代品具有巨大潜力。
