Dai G Z, Sun M S, Liu S Q, Ding X F, Chen Y D, Wang L C, Du D P, Zhao G, Su Y, Li J
J Med Virol. 1987 Aug;22(4):365-73. doi: 10.1002/jmv.1890220409.
An outbreak of diarrhoea in neonates occurred at the nurseries of the Department of Obstetrics of Zhao Tong Regional Hospital, Yunnan Province, from the middle of August to the end of November, 1985. Fifty-one percent of children were affected 2-8 days after birth. The clinical symptoms were mild; patients mainly had diarrhoea and did not vomit. Rotaviruses were detected in 66.7% by RNA PAGE and in 72.7% by EM. The virus strain designated as KMB/R85 had a typical morphology, which was indistinguishable from that of infantile rotaviruses by EM. The viral RNA genome was composed of 11 segments. The buoyant density in CsCl was 1.377 g/cm3. The KMB/R85 strain possessed a hemagglutinin for rhesus monkey erythrocytes. By ELISA, IEM, and HAI, it was found that KMB/R85 strain did not possess the common group antigen shared by group A rotaviruses and was antigenically similar to the Chinese adult diarrhoea rotavirus (serogroup B).
1985年8月中旬至11月底，云南省昭通地区医院产科病房新生儿中发生了腹泻暴发。51%的患儿在出生后2至8天受到影响。临床症状较轻；患者主要表现为腹泻，无呕吐症状。通过RNA聚丙烯酰胺凝胶电泳（RNA PAGE）检测，66.7%的病例检出轮状病毒，通过电子显微镜（EM）检测，72.7%的病例检出轮状病毒。命名为KMB/R85的病毒株具有典型形态，通过电子显微镜观察，其与婴儿轮状病毒无法区分。病毒RNA基因组由11个节段组成。在氯化铯中的浮密度为1.377 g/cm³。KMB/R85株具有针对恒河猴红细胞的血凝素。通过酶联免疫吸附测定（ELISA）、免疫电镜检查（IEM）和血凝抑制试验（HAI）发现，KMB/R85株不具有A组轮状病毒共有的共同群抗原，并且在抗原性上与中国成人腹泻轮状病毒（血清群B）相似。
