Yap K L, Wong Y H, Khor C M, Ooi Y E
Department of Medical Microbiology and Immunology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur.
Can J Microbiol. 1992 Sep;38(9):996-9. doi: 10.1139/m92-162.
A 12-month study was carried out on the molecular epidemiology of rotavirus in urban and suburban Malaysian children. Analysis of faecal samples from 973 hospitalized diarrhoeic children by polyacrylamide gel electrophoresis detected 268 rotaviruses (28%). All isolates were group A rotaviruses, which produced 22 electropherotypes: 16 (91.5%) with long RNA migration patterns and 6 (8.5%) with short patterns. One of the long-pattern electropherotypes was the predominant strain (71.1% of the total electropherotypes) isolated during this study. Although 3 other strains were detected sporadically over the study period, 16 others were present only during the first 7 months and 2 others were confined to the last 5 months. Long- and short-pattern electropherotypes were found to co-circulate extensively. There was a significant association of short-pattern electropherotypes with infection in older children. In addition, the prevalence of vomiting and mean duration of diarrhoea were significantly associated with different electropherotypes.
一项针对马来西亚城市和郊区儿童轮状病毒分子流行病学的为期12个月的研究开展。通过聚丙烯酰胺凝胶电泳对973名住院腹泻儿童的粪便样本进行分析，检测出268例轮状病毒（28%）。所有分离株均为A组轮状病毒，产生了22种电泳型：16种（91.5%）具有长RNA迁移模式，6种（8.5%）具有短模式。其中一种长模式电泳型是本研究期间分离出的主要毒株（占总电泳型的71.1%）。尽管在研究期间还偶尔检测到其他3种毒株，但另外16种仅在前7个月出现，还有2种仅在最后5个月出现。发现长模式和短模式电泳型广泛共同流行。短模式电泳型与大龄儿童感染之间存在显著关联。此外，呕吐发生率和腹泻平均持续时间与不同电泳型显著相关。
