Rivrud G N
Department of Gynaecology and Obstetrics, National Hospital, University of Oslo, Norway.
Mutat Res. 1988 Jul;208(3-4):195-200. doi: 10.1016/0165-7992(88)90060-7.
Small amounts of seminal fluid strongly enhanced the mutagenicity of the precursor mutagen benzo[a]pyrene (BP) in the Salmonella/microsome test. This previously unreported effect was found only in the presence of S9 mix for metabolic activation. The increase far exceeded the additive effect expected from experiments where seminal fluid and BP were tested separately with S9 mix. Testing of the direct-acting mutagen 4-nitro-o-phenylene-diamine (NPD) together with seminal fluid resulted in a lower mutagenic activity than that of NPD alone. Seminal fluid had a bactericidal effect on the Salmonella bacteria, thus only volumes up to 40 microliter could be used per plate. The mutagenic effect of only seminal fluid and S9 mix was slightly increased over controls in a standard Ames test, but was equal to the spontaneous mutation rate with a preincubation test modified according to Kado and coworkers. There were no significant differences between seminal plasma from smokers and non-smokers in any experimental series. Seminal fluid concentrated 20-fold by extraction with the mutagen-removing adsorbant Mutasorb did not have any enhancing effect on the mutagenicity of BP, nor did it exhibit any mutagenic activity in itself with or without S9 mix.
在沙门氏菌/微粒体试验中，少量精液可显著增强前体诱变剂苯并[a]芘（BP）的诱变性。这种先前未报道的效应仅在存在用于代谢活化的S9混合物时才会出现。其增加幅度远远超过了精液和BP分别与S9混合物进行试验时预期的相加效应。将直接作用诱变剂4-硝基邻苯二胺（NPD）与精液一起测试时，诱变活性低于单独使用NPD时的诱变活性。精液对沙门氏菌有杀菌作用，因此每平板只能使用最多40微升的精液。在标准艾姆斯试验中，仅精液和S9混合物的诱变效应比对照略有增加，但在根据卡多及其同事修改的预孵育试验中，该诱变效应与自发突变率相当。在任何实验系列中，吸烟者和非吸烟者的精浆之间均无显著差异。用去除诱变剂的吸附剂Mutasorb萃取浓缩20倍的精液对BP的诱变性没有任何增强作用，并且无论有无S9混合物，其本身均未表现出任何诱变活性。
