Rabah Houem, Ferret-Bernard Stéphanie, Huang Song, Le Normand Laurence, Cousin Fabien J, Gaucher Floriane, Jeantet Romain, Boudry Gaëlle, Jan Gwénaël
STLO, INRA, Agrocampus Ouest, Rennes, France.
Pôle Agronomique Ouest, Rennes, France.
Front Microbiol. 2018 Oct 29;9:2584. doi: 10.3389/fmicb.2018.02584. eCollection 2018.
is a beneficial bacterium, used as a cheese starter, which presents versatile probiotic properties. These properties are strain-dependent. We hypothesized they may also be delivery vehicle-dependent. In this study, we thus explored in healthy piglets how the cheese matrix affects the immunomodulatory properties of . During 2 weeks, three groups of weaned piglets consumed, respectively, as a liquid culture (PF-culture), under the form of a cheese (PF-cheese), or a control sterile cheese matrix (Cheese-matrix). The metabolic activity of was assessed by determining short chain fatty acids (SCFA) concentration and bifidobacteria population in feces. Whatever the delivery vehicle, was metabolically active in piglets' colon and enhanced both bifidobacteria and SCFA in feces. consumption decreased the secretion of TNFα and of IL-10 by peripheral blood mononuclear cells (PBMC). It did not alter IL-10, IFNγ, IL-17, and TNFα secretion in mesenteric lymph node immune cells (MLNC). PF-cheese enhanced significantly Treg phenotype, while PF-culture decreased significantly Th17 phenotype in PBMC and MLNC. Remarkably, only PF-cheese induced an increase of Th2 phenotype in PBMC and MLNC. stimulation of PBMC and MLNC by Lipopolysaccharides and Concanavalin A emphasized the difference in the immunomodulatory responses between PF-culture and PF-cheese group, as well as between PBMC and MLNC. This study shows the importance to consider the delivery vehicle for probiotic administration. It confirms the anti-inflammatory potential of . It opens new perspectives for the use propionibacteria-fermented products as preventive agents for inflammatory bowel diseases and intestinal infectious diseases.
是一种有益细菌，用作奶酪发酵剂，具有多种益生菌特性。这些特性取决于菌株。我们推测它们也可能取决于递送载体。因此，在本研究中，我们在健康仔猪中探索了奶酪基质如何影响的免疫调节特性。在2周内，三组断奶仔猪分别食用作为液体培养物（PF-培养物）、呈奶酪形式的（PF-奶酪）或对照无菌奶酪基质（奶酪基质）。通过测定粪便中的短链脂肪酸（SCFA）浓度和双歧杆菌数量来评估的代谢活性。无论递送载体如何，在仔猪结肠中都具有代谢活性，并增加了粪便中的双歧杆菌和SCFA。食用降低了外周血单核细胞（PBMC）中TNFα和IL-10的分泌。它没有改变肠系膜淋巴结免疫细胞（MLNC）中IL-10、IFNγ、IL-17和TNFα的分泌。PF-奶酪显著增强了PBMC和MLNC中的Treg表型，而PF-培养物显著降低了PBMC和MLNC中的Th17表型。值得注意的是，只有PF-奶酪诱导了PBMC和MLNC中Th2表型的增加。脂多糖和伴刀豆球蛋白A对PBMC和MLNC的刺激强调了PF-培养物组和PF-奶酪组之间以及PBMC和MLNC之间免疫调节反应的差异。本研究表明考虑益生菌给药的递送载体的重要性。它证实了的抗炎潜力。它为使用丙酸杆菌发酵产品作为炎症性肠病和肠道传染病的预防剂开辟了新的前景。
