Development and evaluation of antibacterial electrospun pea protein isolate-polyvinyl alcohol nanocomposite mats incorporated with cinnamaldehyde.

Electrospun film is developed from an electrically charged ultrafine jet of a polymer solution or melt as a matrix of thin/nano fibers struck on to a target surface. The objective of this work was to obtain homogeneous nanofibers from pea protein isolate (PPI) in polyvinyl alcohol (PVA) by hybrid electrospinning as well as incorporating cinnamaldehyde (CA) into the matrix to obtain an antibacterial mat. The effect of processing conditions, pH, polymer and CA concentrations on formulation properties and nanofiber morphology were investigated and the mats were visualized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Rheological evaluation indicated a pseudoplastic behavior for all formulations. Alkaline pH formulation led to a decreasing apparent viscosity and an increasing electrical conductivity resulting in the formation of more homogeneous fibers. The 50:50 mass percentage ratio of PPI/PVA solutions produced homogeneous nanofibers with the average fiber diameter of 485 ± 85 nm. FTIR spectroscopy confirmed uniform dispersion of PPI and PVA. The minimum concentration of CA to inhibit both Gram negative and Gram positive bacteria was 1%. The average diameter of nanofibers decreased from 257 ± 51 nm to 219 ± 31 nm by increasing CA content from 0.25 to 1.5%.