Molecular Endocrinology Laboratory (KMEB), Department of Endocrinology, Odense University Hospital & University of Southern Denmark, Odense, Denmark; Department of Orthodontics and Dentofacial Orthopaedics, Amrita School of Dentistry, Amrita Vishwa Vidyapeetham, Kochi, Kerala 682041, India.
Division of Tissue Engineering and Regeneration Technologies (DTERT), Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, India.
Mater Sci Eng C Mater Biol Appl. 2019 Jan 1;94:509-515. doi: 10.1016/j.msec.2018.09.054. Epub 2018 Sep 25.
Drug functionalized scaffolds are currently being employed to improve local delivery of osteoprotective drugs with the aim of reducing their loading dose as well as unwanted systemic complications. In this study we tested a poly-(ε) caprolactone (PCL)-laponite-strontium ranelate (SRA) composite scaffold (PLS3) for its abilities to support growth and osteogenic differentiation of human marrow derived stromal stem cells (hMSC). The in vitro experiments showed the PLS3 scaffold supported cell growth and osteogenic differentiation. The in vivo implantation of hMSC seeded PLS3 scaffold in immunocompromised mice revealed vascularized ectopic bone formation. PLS3 scaffolds can be useful in bone regenerative applications in the fields of orthopaedics and dentistry.
药物功能化支架目前被用于改善骨保护药物的局部递送，目的是减少其载药量和避免不必要的全身并发症。在这项研究中，我们测试了一种聚（ε）己内酯（PCL）-膨润土-雷奈酸锶（SRA）复合支架（PLS3），以评估其支持人骨髓基质干细胞（hMSC）生长和成骨分化的能力。体外实验表明，PLS3 支架支持细胞生长和成骨分化。将 hMSC 接种在 PLS3 支架上植入免疫缺陷小鼠体内，可观察到血管化异位骨形成。PLS3 支架可用于骨科和牙科领域的骨再生应用。
