Mohaghegh Sadra, Nokhbatolfoghahaei Hanieh, Baniameri Sahar, Farajpour Hekmat, Fakhr Massoumeh Jabbari, Shokrolahi Fatemeh, Khojasteh Arash
Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Artificial Intelligence, Smart University of Medical Sciences, Tehran, Iran.
Int J Biomater. 2024 Aug 19;2024:1365080. doi: 10.1155/2024/1365080. eCollection 2024.
Bone tissue engineering necessitates the development of scaffolds with optimal properties to provide a suitable microenvironment for cell adhesion, proliferation, and osteogenic differentiation. The selection of appropriate scaffold materials remains a critical challenge in this field. In this study, we aimed to address this challenge by evaluating and comparing the performance of hydrogel scaffolds reinforced with -tricalcium phosphate (-TCP), allograft, and a combination of allograft and strontium hydroxyapatite (SrHA). In this study, scaffolds containing the following compounds with a weight ratio of 75 : 25 : 50 were made using a 3D printer: group (1) alginate + gelatin + -TCP (TCP), group (2) alginate + gelatin + allograft (Allo), and group (3) alginate + gelatin + allograft + strontium hydroxyapatite (Str). Stem cells extracted from rat bone marrow (rBMSCs) were cultured on scaffolds, and cell proliferation and differentiation tests were performed. Also, the physical and chemical properties of the scaffolds were investigated. The two/one-way analysis of variance (ANOVA) by Tukey's post hoc test was performed. There was no significant difference between scaffolds with pore size and porosity. TCP scaffolds' mechanical strength and degradation rate were significantly lower than the other two groups ( < 0.05). Also, the swelling ratio of Allo scaffolds was higher than in other samples. The amount of cell proliferation in the samples of the TCP group was lower than the other two, and the Allo samples had the best results in this concern ( < 0.01). However, the scaffolds containing strontium hydroxyapatite had significantly higher bone differentiation compared to the other two groups, and the lowest results were related to the scaffolds containing -TCP. Hydrogel scaffolds reinforced with allograft or its combination with strontium showed better physicochemical and biological behavior compared to those reinforced with -TCP. Besides, adding strontium had a limited impact on the physicochemical features of allograft-containing scaffolds while improving their potential to induce osteogenic differentiation.
骨组织工程需要开发具有最佳性能的支架，以为细胞黏附、增殖和成骨分化提供合适的微环境。选择合适的支架材料仍然是该领域的一项关键挑战。在本研究中，我们旨在通过评估和比较用β-磷酸三钙（β-TCP）、同种异体移植物以及同种异体移植物与羟基锶磷灰石（SrHA）的组合增强的水凝胶支架的性能来应对这一挑战。在本研究中，使用3D打印机制作了含有以下重量比为75∶25∶50的化合物的支架：组（1）藻酸盐+明胶+β-TCP（TCP），组（2）藻酸盐+明胶+同种异体移植物（Allo），以及组（3）藻酸盐+明胶+同种异体移植物+羟基锶磷灰石（Str）。将从大鼠骨髓（rBMSCs）中提取的干细胞接种在支架上，并进行细胞增殖和分化测试。此外，还研究了支架的物理和化学性质。采用Tukey事后检验进行双因素/单因素方差分析（ANOVA）。孔径和孔隙率方面，各支架之间无显著差异。TCP支架的机械强度和降解率显著低于其他两组（P<0.05）。此外，Allo支架的溶胀率高于其他样品。TCP组样品中的细胞增殖量低于其他两组，在这方面Allo样品的结果最佳（P<0.01）。然而，与其他两组相比，含有羟基锶磷灰石的支架具有显著更高的骨分化，而最低结果与含有β-TCP的支架有关。与用β-TCP增强的水凝胶支架相比，用同种异体移植物或其与锶的组合增强的水凝胶支架表现出更好的物理化学和生物学行为。此外，添加锶对含同种异体移植物的支架的物理化学特征影响有限，同时提高了它们诱导成骨分化的潜力。
