a School of Preclinical Medicine , Beijing University of Chinese Medicine , Beijing , China.
b School of Chinese Materia Medica , Beijing University of Chinese Medicine , Beijing , China.
Artif Cells Nanomed Biotechnol. 2018;46(sup3):S308-S317. doi: 10.1080/21691401.2018.1492419. Epub 2018 Nov 15.
Schizonepetae Spica Carbonisata (SSC) has pronounced haemostatic effects for hundreds of years and has been acknowledged in the 2015 Pharmacopoeia of the People's Republic of China (PPRC) as a haemostatic charcoal drug. However, after years of efforts, the underlying mechanism and the material basis is still less defined. In this research, we developed a novel CDs derived from SSC (SSC-CDs) with an average diameter of 1.29-6.87 nm and a quantum yield of 6.31%. SSC was prepared using a modified pyrolysis method and no further modification and external surface passivation agent is required. With abundant surface groups, SSC-CDs showed distinct solubility and bioactivity. In this study, we innovatively used the Deinagkistrodon acutus (D. acutus) venom model as well as the classical haemorrhagic animal model to evaluate the haemostatic bioactivity of SSC-CDs. The results indicated that SSC-CDs had outstanding haemostatic bioactivity and might inhibit the haemorrhagic activity via PLT elevation. According to the results of this study and our previous work, we discovered that CDs derived from different kinds of charcoal drugs presented similarities and differences in the structural feature, physicochemical property and bioactivity. In order to further explore the self-bioactivities, we first named this kind of CDs as "Chinese Medicine charcoal drug nanoparticles" (CMNP). These results may not only provide evidence for further researches of self-bioactivities of CDs but give new insights into potential biomedical and healthcare applications of CDs, therefore, make contributions to future drug discovery.
荆芥炭（SSC）数百年来具有显著的止血作用，并在 2015 年版《中华人民共和国药典》（PPRC）中被认可为一种止血炭药。然而，经过多年的努力，其潜在机制和物质基础仍不明确。在这项研究中，我们开发了一种新型的来源于荆芥的碳点（SSC-CDs），其平均直径为 1.29-6.87nm，量子产率为 6.31%。SSC 是通过改良的热解方法制备的，不需要进一步的修饰和外部表面钝化剂。由于含有丰富的表面基团，SSC-CDs 具有明显的溶解性和生物活性。在本研究中，我们创新性地采用尖吻蝮蛇（D. acutus）蛇毒模型以及经典的出血动物模型来评估 SSC-CDs 的止血生物活性。结果表明，SSC-CDs 具有优异的止血生物活性，可能通过血小板升高来抑制出血活性。根据本研究和我们之前的工作结果，我们发现来源于不同炭药的碳点在结构特征、理化性质和生物活性方面既有相似之处，也有不同之处。为了进一步探索其自生物活性，我们首先将这类碳点命名为“中药炭药纳米颗粒”（CMNP）。这些结果不仅可为碳点自生物活性的进一步研究提供证据，也为碳点在生物医学和医疗保健方面的潜在应用提供了新的思路，因此，为未来的药物发现做出了贡献。
