Larrabee W F, Bolen J W, Sutton D
Section of Otolaryngology, Virginia Mason Clinic, Seattle.
Arch Otolaryngol Head Neck Surg. 1988 Sep;114(9):982-6. doi: 10.1001/archotol.1988.01860210048013.
Myofibroblasts in human granulation tissue have many of the structural and functional characteristics of smooth-muscle cells and appear to be responsible for wound contraction. They have also been identified in contracted scar tissue in nongranulation wounds. In this report, their role in head and neck wound healing will be explored utilizing transmission electron microscopy and immunoperoxidase techniques with antibodies to the intermediate filament vimentin and to muscle-restricted actins. In piglets, high-tension, low-tension, and granulating wounds were created and studied with serial biopsy specimens. Results showed few myofibroblasts in either the high- or low-tension wounds and multiple myofibroblasts in the granulating wounds. In the clinical studies, the immunoperoxidase technique with monoclonal antibody to muscle-specific actins proved most useful in identifying myofibroblasts. Myofibroblasts were present in granulating wounds and hypertrophic scars. They were not widely present in mature keloids.
人肉芽组织中的肌成纤维细胞具有许多平滑肌细胞的结构和功能特征，似乎与伤口收缩有关。在非肉芽伤口的收缩瘢痕组织中也发现了它们。在本报告中，将利用透射电子显微镜和免疫过氧化物酶技术，使用针对中间丝波形蛋白和肌肉特异性肌动蛋白的抗体，探讨它们在头颈部伤口愈合中的作用。在仔猪身上制造了高张力、低张力和肉芽伤口，并对连续活检标本进行了研究。结果显示，高张力或低张力伤口中的肌成纤维细胞很少，而肉芽伤口中有多个肌成纤维细胞。在临床研究中，使用针对肌肉特异性肌动蛋白的单克隆抗体的免疫过氧化物酶技术在识别肌成纤维细胞方面最为有用。肌成纤维细胞存在于肉芽伤口和肥厚性瘢痕中。它们在成熟瘢痕疙瘩中并不广泛存在。
