Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
National Blood Center, Bangkok, Thailand.
Transfusion. 2019 Mar;59(3):1035-1043. doi: 10.1111/trf.15041. Epub 2018 Nov 15.
Hepatitis E virus (HEV) infection in several industrialized and developing countries is associated with the consumption of pork and other meat products, an exposure risk among the majority of blood donors. We aimed to evaluate the prevalence of HEV in plasma from healthy blood donors in Thailand.
We screened blood samples collected between October and December 2015, from 30,115 individual blood donors in 5020 pools of six, for HEV RNA using in-house real-time reverse-transcription polymerase chain reaction (RT-PCR). Thrice-reactive samples were subjected to a commercial real-time RT-PCR (cobas HEV test) and evaluated for anti-HEV immunoglobulin M and immunoglobulin G antibodies. Genotyping using nested RT-PCR, nucleotide sequencing, and phylogenetic analysis was performed.
Twenty-six donors were positive for HEV RNA by the in-house assay, nine of whom were also positive by cobas test. None of the latter were reactive for anti-HEV immunoglobulin M or immunoglobulin G antibodies. Six samples were successfully genotyped and found to be HEV genotype 3. Thus, the frequency of HEV infection among healthy Thai blood donors is 1 in 1158.
The presence of HEV RNA in the Thai blood supply was comparable to the rates reported in western European countries, but higher than in North America and Australia.
在一些工业化和发展中国家，戊型肝炎病毒（HEV）感染与食用猪肉和其他肉类产品有关，而这是大多数献血者面临的暴露风险。我们旨在评估泰国健康献血者血浆中 HEV 的流行率。
我们使用内部实时逆转录聚合酶链反应（RT-PCR），对 2015 年 10 月至 12 月间采集的 5020 个 6 人份血样池中的 30115 名个体献血者的血液样本进行了 HEV RNA 筛查。对 3 次反应性样本进行了商业实时 RT-PCR（cobas HEV 检测），并评估了抗 HEV 免疫球蛋白 M 和免疫球蛋白 G 抗体。使用巢式 RT-PCR、核苷酸测序和系统发育分析进行了基因分型。
26 名献血者通过内部检测呈 HEV RNA 阳性，其中 9 名献血者通过 cobas 检测也呈阳性。后者均未对抗 HEV 免疫球蛋白 M 或免疫球蛋白 G 抗体产生反应。成功对 6 个样本进行了基因分型，发现它们均为 HEV 基因型 3。因此，泰国健康献血者中 HEV 感染的频率为 1/1158。
泰国血液供应中存在 HEV RNA，其水平与西欧国家报告的水平相当，但高于北美和澳大利亚。
