Nielsen P B, Mojon M
Statens Seruminstitut, Department of Diagnostic Bacteriology, Copenhagen, Denmark.
APMIS. 1988 Jul;96(7):649-54. doi: 10.1111/j.1699-0463.1988.tb00924.x.
An ELISA to measure Pneumocystis carinii-specific IgG, IgM, and IgA has been developed. The antigen was prepared from purified cysts by sonication and ultracentrifugation. Antigen particles with sedimentation coefficients less than 165 S were used. The technique has been compared with indirect immunofluorescence, using whole cysts as antigen. Ninety human sera were assayed. The results were significantly correlated. The ELISA-technique was more sensitive, and owing to the soluble antigen the daily variation was less than 1 per cent. The technique is useful for quick and reliable detection of Pneumocystis carinii antibodies in a routine laboratory.
已开发出一种用于检测卡氏肺孢子虫特异性IgG、IgM和IgA的酶联免疫吸附测定法（ELISA）。抗原是通过超声处理和超速离心从纯化的包囊中制备的。使用沉降系数小于165 S的抗原颗粒。该技术已与以完整包囊为抗原的间接免疫荧光法进行了比较。检测了90份人血清。结果具有显著相关性。ELISA技术更灵敏，并且由于使用可溶性抗原，每日变化小于1%。该技术对于在常规实验室中快速可靠地检测卡氏肺孢子虫抗体很有用。
