Department of Cell Biology, University Medical Center Rostock, Rostock, Germany.
Cell Biol Int. 2019 Jan;43(1):22-32. doi: 10.1002/cbin.11073.
One of the most popular cell lines in osteogenesis studies is the human osteoblastic line MG-63. For cell biological investigation, it is important that the cells remain stable in their phenotype over several passages in cell culture. MG-63 cells can be used to provide fundamental insights into cell--material interaction. The aim of this study is to present a systematic characterization of the physiological behavior of MG-63 cells in the range of passages 5-30. Significant cell physiology processes during the first 24 h, including cell morphology, availability of adhesion receptors, cell cycle phases, as well as the expression of the signaling proteins Akt, GSK3a/b, IkB-α, ERK1/2, p38-MAPK, and intracellular calcium ion mobilization, remained stable over the entire range of passages P5-P30. Due to these stable characteristics in a wide range of cell culture passages, MG-63 cells can be considered as a suitable in vitro model to analyze the biocompatibility and biofunctionality of implant materials.
在成骨研究中，最受欢迎的细胞系之一是人成骨样细胞系 MG-63。对于细胞生物学研究，重要的是细胞在细胞培养中经过多次传代后仍能保持其表型稳定。MG-63 细胞可用于提供对细胞-材料相互作用的基本了解。本研究的目的是系统表征 MG-63 细胞在 5-30 代范围内的生理行为。在最初的 24 小时内，包括细胞形态、粘附受体的可利用性、细胞周期阶段以及信号蛋白 Akt、GSK3a/b、IkB-α、ERK1/2、p38-MAPK 的表达和细胞内钙离子动员等重要的细胞生理过程，在整个 P5-P30 代范围内保持稳定。由于在广泛的细胞培养代范围内具有这些稳定的特性，MG-63 细胞可被视为一种合适的体外模型，用于分析植入材料的生物相容性和生物功能性。
