Dpto. de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, Spain; CIBER de Bioingeniería Biomateriales y Nanomedicina (CIBER-BBN), Spain.
CIBER de Bioingeniería Biomateriales y Nanomedicina (CIBER-BBN), Spain; Dpto. de Biología Celular, Genética y Fisiología, Instituto de Investigación Biomédica de Málaga (IBIMA), Universidad de Málaga, Spain; Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), c/ Severo Ochoa 35, 29590 Campanillas, Málaga, Spain.
Acta Biomater. 2019 Jan 1;83:456-466. doi: 10.1016/j.actbio.2018.11.017. Epub 2018 Nov 13.
The osteogenic and angiogenic responses to metal macroporous scaffolds coated with silicon substituted hydroxyapatite (SiHA) and decorated with vascular endothelial growth factor (VEGF) have been evaluated in vitro and in vivo. Ti6Al4V-ELI scaffolds were prepared by electron beam melting and subsequently coated with Ca(PO)(SiO)(OH) following a dip coating method. In vitro studies demonstrated that SiHA stimulates the proliferation of MC3T3-E1 pre-osteoblastic cells, whereas the adsorption of VEGF stimulates the proliferation of EC mature endothelial cells. In vivo studies were carried out in an osteoporotic sheep model, evidencing that only the simultaneous presence of both components led to a significant increase of new tissue formation in osteoporotic bone. STATEMENT OF SIGNIFICANCE: Reconstruction of bones after severe trauma or tumors extirpation is one of the most challenging tasks in the field of orthopedic surgery. This scenario is even more complicated in the case of osteoporotic patients, since their bone regeneration capability is decreased. In this work we present a porous implant that promotes bone regeneration even in osteoporotic bone. By coating the implant with osteogenic bioceramics such as silicon substituted hydroxyapatite and subsequent adsorption of vascular endothelial growth factor, these implants stimulate the bone ingrowth when they are implanted in osteoporotic sheep.
已对涂覆有硅取代羟基磷灰石（SiHA）并修饰有血管内皮生长因子（VEGF）的金属大孔支架的成骨和成血管反应进行了体外和体内评估。通过电子束熔化制备 Ti6Al4V-ELI 支架，随后通过浸涂法涂覆 Ca(PO)(SiO)(OH)。体外研究表明 SiHA 刺激 MC3T3-E1 前成骨细胞的增殖，而 VEGF 的吸附刺激 EC 成熟内皮细胞的增殖。在骨质疏松绵羊模型中进行了体内研究，证明只有同时存在这两种成分才能导致骨质疏松骨中新组织形成的显著增加。 意义声明：在矫形外科领域，严重创伤或肿瘤切除后的骨骼重建是最具挑战性的任务之一。对于骨质疏松症患者来说，情况更加复杂，因为他们的骨骼再生能力下降。在这项工作中，我们提出了一种多孔植入物，即使在骨质疏松症的骨骼中也能促进骨骼再生。通过在植入物上涂覆骨生成生物陶瓷，如硅取代羟基磷灰石，并随后吸附血管内皮生长因子，当这些植入物被植入骨质疏松症绵羊时，它们会刺激骨内生长。
