Laboratory of Enzyme Chemistry, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok, Russian Federation.
Carbohydr Polym. 2019 Feb 1;205:465-471. doi: 10.1016/j.carbpol.2018.10.083. Epub 2018 Oct 29.
Fucoidan from brown alga Fucus evanescens and its product of enzymatic hydrolysis have precisely established structure and possess significant biological activities. The aim of present study was to determine radiosensitizing activity of fucoidan from brown alga F. evanescens and its derivative in human melanoma, breast adenocarcinoma, and colorectal carcinoma cell lines and elucidate mechanism of their action. The fucoidan from F. evanescens and its derivative had a comparable radiosensitizing activity and increased the inhibiting effect of X-ray radiation on proliferation and colony formation of human cancer cells, with significant inhibition of melanoma cells. The molecular mechanism of this action was associated with the induction of apoptosis by activating the initiator and effector caspases, suppressing the expression of the anti-apoptotic protein, and enhancing the fragmentation of DNA. The obtained data confirm the prospects of using fucoidan's derivative in combination with radiation therapy for the improvement of the schemes of cancer therapy.
来自褐藻泡叶藻的岩藻聚糖及其酶解产物具有明确的结构，并具有显著的生物学活性。本研究的目的是确定来自褐藻泡叶藻的岩藻聚糖及其衍生物在人黑色素瘤、乳腺腺癌和结肠直肠癌细胞系中的放射增敏活性，并阐明其作用机制。泡叶藻岩藻聚糖及其衍生物具有相当的放射增敏活性，增加了 X 射线辐射对人癌细胞增殖和集落形成的抑制作用，对黑色素瘤细胞有显著的抑制作用。这种作用的分子机制与通过激活起始和效应半胱天冬酶诱导细胞凋亡、抑制抗凋亡蛋白的表达以及增强 DNA 片段化有关。所得数据证实了使用岩藻聚糖衍生物与放射治疗联合应用于改善癌症治疗方案的前景。
