Department of Applied Biology & Chemical Technology, State Key Laboratory of Chinese Medicine and Molecular Pharmacology in Shenzhen, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
College of Agricultural Resource and Environment, Heilongjiang University, 15000 Harbin, China.
Carbohydr Polym. 2016 Feb 10;137:739-746. doi: 10.1016/j.carbpol.2015.05.061. Epub 2015 Jun 4.
This study aims to discover novel and bioactive polysaccharides (PS) from wild Armillaria ostoyae, a honey mushroom species. Two PS designated AkPS1V-1 (66.6 kDa) and AkPS1V-2 (15.3 kDa) were isolated and fractionated by anion ion exchange (IEC) and size exclusion chromatography (SEC) from the alkaline extract of A. ostoyae mushrooms. AkPS1V-1 was a glucan composed of solely glucose residues and AkPS1V-2 a heteropolysaccharide composed of glucose and galactose at 6:1 molar ratio. AkPS1V-2 exhibited higher antioxidant activities than AkPS1V-1 based on reducing power, radical scavenging and metal chelating assays. The structure of AkPS1V-2 was further analyzed and elucidated as a branched galactoglucan with a backbone composed of (1→6)-β-D-glucopyranosyl, (1→3)-β-D-glucopyranosyl, (1→3)-α-D-galactopyranosyl and (1→3,6)-β-D-glucopyranosyl residues at 3:1:1:1 ratio, and side chain of (1→3)-β-D-glucopyranosyl residue. This is the first report on a pure PS structure and its antioxidant activities from this mushroom species.
本研究旨在从野生蜜环菌（一种蜂蜜蘑菇）中发现新型生物活性多糖（PS）。两种多糖 AkPS1V-1（66.6 kDa）和 AkPS1V-2（15.3 kDa）通过阴离子交换（IEC）和尺寸排阻色谱（SEC）从蜜环菌碱性提取物中分离和分级。AkPS1V-1 是一种由纯葡萄糖残基组成的葡聚糖，而 AkPS1V-2 是一种由葡萄糖和半乳糖以 6:1 摩尔比组成的杂多糖。基于还原能力、自由基清除和金属螯合测定，AkPS1V-2 表现出比 AkPS1V-1 更高的抗氧化活性。进一步分析和阐明了 AkPS1V-2 的结构，确定它是一种支链半乳糖葡聚糖，其主链由（1→6）-β-D-吡喃葡萄糖基、（1→3）-β-D-吡喃葡萄糖基、（1→3）-α-D-吡喃半乳糖基和（1→3,6）-β-D-吡喃葡萄糖基残基以 3:1:1:1 的比例组成，侧链由（1→3）-β-D-吡喃葡萄糖基残基组成。这是首次从该蘑菇物种中报道纯 PS 结构及其抗氧化活性。
