Puca Annibale A, Ferrario Anna, Maciag Anna, Accardi Giulia, Aiello Anna, Gambino Caterina Maria, Candore Giuseppina, Caruso Calogero, Namboodiri Aryan M, Pandey Janardan P
1Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
2IRCCS MultiMedica, Milan, Italy.
Immun Ageing. 2018 Nov 6;15:26. doi: 10.1186/s12979-018-0134-7. eCollection 2018.
The aim of this study was to analyse the role of GM allotypes, i.e. the hereditary antigenic determinants expressed on immunoglobulin polypeptide chains, in the attainment of longevity. The role played by immunoglobulin allotypes in the control of immune responses is well known as well as the role of an efficient immune response in longevity achievement. So, it is conceivable that particular GM allotypes may contribute to the generation of an efficient immune response that supports successful ageing, hence longevity.
In order to show if GM allotypes play a role in the achievement of longevity, we typed the DNA of 95 Long-living individuals (LLIs) and 96 young control individuals (YCs) from South Italy for GM3/17 and GM23+/- alleles.
To demonstrate the role of GM allotypes in the attainment of longevity we compared genotype and allele frequencies of GM allotypes between LLIs and YCs. A global chi-square test (3 × 2) shows that the distribution of genotypes at the GM 3/17 locus is highly significantly different in LLIs from that observed in YCs ( < 0.0001). The 2 × 2 chi-square test shows that the carriers of the GM3 allele contribute to this highly significant difference. Accordingly, GM3 allele is significantly overrepresented in LLIs. No significant differences were instead observed regarding GM23 allele.
These preliminary results show that GM3 allotype is significantly overrepresented in LLIs. To best of our knowledge, this is the first study performed to assess the role of GM allotypes in longevity. So, it should be necessary to verify the data in a larger sample of individuals to confirm GM role in the attainment of longevity.
本研究的目的是分析免疫球蛋白同种异型（即免疫球蛋白多肽链上表达的遗传性抗原决定簇）在长寿过程中的作用。免疫球蛋白同种异型在免疫反应控制中所起的作用以及有效免疫反应在实现长寿中的作用是众所周知的。因此，可以想象特定的免疫球蛋白同种异型可能有助于产生支持成功衰老从而实现长寿的有效免疫反应。
为了表明免疫球蛋白同种异型是否在长寿过程中发挥作用，我们对来自意大利南部的95名长寿个体（LLIs）和96名年轻对照个体（YCs）的DNA进行了GM3/17和GM23+/-等位基因分型。
为了证明免疫球蛋白同种异型在长寿过程中的作用，我们比较了LLIs和YCs之间免疫球蛋白同种异型的基因型和等位基因频率。全局卡方检验（3×2）表明，GM 3/17位点的基因型分布在LLIs中与在YCs中观察到的情况有极显著差异（<0.0001）。2×2卡方检验表明，GM3等位基因的携带者促成了这种极显著差异。因此，GM3等位基因在LLIs中显著过量存在。相反，关于GM23等位基因未观察到显著差异。
这些初步结果表明，GM3同种异型在LLIs中显著过量存在。据我们所知，这是第一项评估免疫球蛋白同种异型在长寿中作用的研究。因此，有必要在更大的个体样本中验证数据，以确认GM在长寿过程中的作用。
