Shen Xueqing, Guo Miaomiao, Yu Haiyuan, Liu Dan, Lu Zhi, Lu Yanhua
a State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai , People's Republic of China.
b Technology Center , Shanghai Inoherb Co. Ltd , Shanghai , People's Republic of China.
Biosci Biotechnol Biochem. 2019 Mar;83(3):561-568. doi: 10.1080/09168451.2018.1547627. Epub 2018 Nov 19.
Madecassoside is a major pentacyclic triterpene saponin from Centella asiatica with multiple pharmaceutical activities. In this study, we focused on its Propionibacterium acnes related anti-inflammation and skin hydration activities, both of which play important roles in skin homeostasis and barrier function. Madecassoside significantly inhibited the pro-inflammatory cytokine IL-1β, TLR2 and nuclear translocation of NF-κB in P. acnes stimulated THP-1 human monocytic cells. In addition, madecasssoside exhibited significant effects on enhancement of skin hydration through increasing the key moisturizing contributors of aquaporin-3, loricrin and involucrin in HaCaT keratinocytes as well as hyaluronan (HA) secretion in human dermal fibroblasts. The upregulation of HA synthases (HAS1, HAS2, HAS3) and inhibition to ROS formation accounted for the increment of HA content. Together, the in vitro study implied the potential medical and cosmetic application of madecassoside in skin protection.
积雪草苷是来自积雪草的一种主要的五环三萜皂苷，具有多种药理活性。在本研究中，我们重点关注其与痤疮丙酸杆菌相关的抗炎和皮肤保湿活性，这两种活性在皮肤稳态和屏障功能中均发挥重要作用。积雪草苷显著抑制痤疮丙酸杆菌刺激的THP-1人单核细胞中促炎细胞因子IL-1β、TLR2以及NF-κB的核转位。此外，积雪草苷通过增加HaCaT角质形成细胞中水通道蛋白-3、兜甲蛋白和内披蛋白的关键保湿成分以及人皮肤成纤维细胞中透明质酸（HA）的分泌，对增强皮肤保湿表现出显著作用。HA合成酶（HAS1、HAS2、HAS3）的上调和对ROS形成的抑制导致了HA含量的增加。总之，体外研究表明积雪草苷在皮肤保护方面具有潜在的医学和美容应用价值。
