Kandasamy Anbazhakan, Aruchamy Kanakaraj, Rangasamy Praveena, Varadhaiyan Deepha, Gowri Chandrasekar, Oh Tae Hwan, Ramasundaram Subramaniyan, Athinarayanan Balasankar
Department of Physics, Gobi Arts & Science College, Gobichettipalayam, Erode 638453, India.
School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Republic of Korea.
Plants (Basel). 2023 Oct 12;12(20):3547. doi: 10.3390/plants12203547.
Centella asiatica (CA) is a medicinal plant widely used in the East, with many of its phytoconstituents remaining unexplored. In this study, compounds were extracted and identified from to determine its medicinal properties. Phytochemical screening was conducted on shoot, callus, and cell suspension extracts, revealing the presence of tannins, flavonoids, terpenoids, saponins, and steroids in all three cultures, with no alkaloids detected. IC50 values were determined to evaluate the antioxidant activity of the extracts, with the highest value observed for cell suspension culture (20 µg/mL), followed by shoot culture (19 µg/mL), and then callus extract (10 µg/mL), with ascorbic acid as the standard at an IC50 value of 26.25 µg/mL. Finally, density functional theory was used to analyze the structure-activity relationships of the identified compounds from extract. The results suggest that ultrasonic-assisted extraction yielded the highest recovery and antioxidant activity, with a scavenging activity of 79%. This study provides valuable insights into the phytochemical composition and antioxidant potential of , which may have implications for its use in traditional medicine and future drug development.
积雪草是一种在东方广泛使用的药用植物，其许多植物成分仍未被探索。在本研究中，从积雪草中提取并鉴定化合物以确定其药用特性。对茎、愈伤组织和细胞悬浮液提取物进行了植物化学筛选，结果显示在所有三种培养物中均存在单宁、黄酮类化合物、萜类化合物、皂苷和甾体，未检测到生物碱。测定IC50值以评估提取物的抗氧化活性，以抗坏血酸为标准，其IC50值为26.25μg/mL，细胞悬浮培养物的IC50值最高（20μg/mL），其次是茎培养物（19μg/mL），然后是愈伤组织提取物（10μg/mL）。最后，利用密度泛函理论分析了积雪草提取物中鉴定出的化合物的构效关系。结果表明，超声辅助提取的回收率和抗氧化活性最高，清除活性为79%。本研究为积雪草的植物化学成分和抗氧化潜力提供了有价值的见解，这可能对其在传统医学中的应用和未来药物开发具有重要意义。
