Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China; QingYunTang Biotech (Beijing) Co., Ltd. Beijing 100176, China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, People's Republic of China.
Int J Biol Macromol. 2019 Mar 1;124:469-477. doi: 10.1016/j.ijbiomac.2018.11.156. Epub 2018 Nov 16.
This study investigated the effects of different temperatures on structural characterization and antitumor activity of polysaccharides from Astragalus membranaceus. APS4 and APS90 were extracted at 4°C and 90°C, respectively, and purified by Sephadex G-200 column. APS4-90 were obtained from APS4 after treatment at 90°C for 6h. MTT results showed that APS4 possessed the highest inhibitory effects on MGC-803, A549 and HepG2 cells. HPGPC analysis showed that the average molecular weights of these polysaccharides were approximately 1.5×10Da, while the asymmetrical peak of APS4-90 suggested heat degradation and configuration changes of APS4. GC, NMR and methylation results showed that these three polysaccharides had similar monosaccharide components (mainly contain glucose), and their backbones were composed of (1→2)‑α‑d‑Glcp. However, APS4 showed higher content of (1→2,6)‑α‑d‑Glcp compared to APS4-90 and APS90, which indicated that higher branched degree would be responsible for the stronger in vitro antitumor activity in APS4. These results were also confirmed by specific rotation and SEM analysis. Our study suggested that APS4 had the potential application for cancer treatment.
本研究考察了不同温度对黄芪多糖结构特征和抗肿瘤活性的影响。APS4 和 APS90 分别在 4°C 和 90°C 下提取，并通过 Sephadex G-200 柱进行纯化。APS4-90 是通过在 90°C 下处理 6h 后从 APS4 中获得的。MTT 结果表明，APS4 对 MGC-803、A549 和 HepG2 细胞具有最高的抑制作用。HPGPC 分析表明，这些多糖的平均分子量约为 1.5×10^4Da，而 APS4-90 的不对称峰表明 APS4 发生了热降解和构象变化。GC、NMR 和甲基化结果表明，这三种多糖具有相似的单糖组成（主要含有葡萄糖），其骨架由（1→2）‑α‑d‑Glcp 组成。然而，与 APS4-90 和 APS90 相比，APS4 具有更高含量的（1→2,6）‑α‑d‑Glcp，这表明更高的支化度是 APS4 具有更强体外抗肿瘤活性的原因。这些结果也通过比旋光度和 SEM 分析得到了证实。我们的研究表明，APS4 具有治疗癌症的潜力。
