Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing 400715, China; Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing 400715, China.
Gene. 2019 Mar 10;688:71-83. doi: 10.1016/j.gene.2018.11.039. Epub 2018 Nov 16.
The cellulose synthase gene superfamily, which includes the cellulose synthase (CesA) and cellulose synthase-like (Csl) gene families, plays a vital role in the biosynthesis of cellulose and hemicellulose in plants. However, these genes have not been extensively studied in tomato (Solanum lycopersicum), a model for Solanaceae plants and for fleshy fruit development. Here, we identified and systematically analyzed 38 CesA/Csl family members that contained cellulose synthase domain regions, and categorized their encoded proteins into 6 subfamilies (CesA, CslA, CslB, CslD, CslE, and CslG) based on phylogenetic analysis. Most CesA/Csl genes from tomato are closely related to those from Arabidopsis, but the families have distinct features regarding gene structure, chromosome distribution and localization, phylogeny, and deduced protein sequence, indicating that they arose via different evolutionary process. Furthermore, expression analysis of CesA/Csl genes in different tissues at various developmental stages showed that most CesAs were constitutively expressed with differential expression levels in various organs; three CslD genes were expressed specifically in flowers, and four CesA and five Csl putative genes were preferentially expressed in fruits. Our results provide insight into the general characteristics of the CesA/Csl genes in tomato, and lay the foundation for further functional studies of CesA/Csl genes in tomato and other Solanaceae species.
纤维素合酶基因超家族，包括纤维素合酶（CesA）和纤维素合酶样（Csl）基因家族，在植物中纤维素和半纤维素的生物合成中起着至关重要的作用。然而，这些基因在番茄（Solanum lycopersicum）中尚未得到广泛研究，番茄是茄科植物和肉质果实发育的模式植物。在这里，我们鉴定并系统分析了 38 个包含纤维素合酶结构域的 CesA/Csl 家族成员，并根据系统发育分析将其编码蛋白分为 6 个亚家族（CesA、CslA、CslB、CslD、CslE 和 CslG）。番茄中的大多数 CesA/Csl 基因与拟南芥中的基因密切相关，但在基因结构、染色体分布和定位、系统发育以及推导的蛋白质序列方面，这些家族具有明显的特征，表明它们是通过不同的进化过程产生的。此外，在不同发育阶段的不同组织中对 CesA/Csl 基因的表达分析表明，大多数 CesA 基因在不同器官中持续表达，表达水平存在差异；三个 CslD 基因在花中特异性表达，四个 CesA 和五个 Csl 推定基因在果实中优先表达。我们的研究结果为番茄中 CesA/Csl 基因的一般特征提供了深入了解，并为进一步研究番茄和其他茄科植物中 CesA/Csl 基因的功能奠定了基础。
