Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria.
Division of Macromolecular Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, 1060 Vienna, Austria.
Nutrients. 2018 Nov 16;10(11):1775. doi: 10.3390/nu10111775.
Our diet is known to substantially influence the immune response not only by support of mucosal barriers but also via direct impact on immune cells. Thus, it was of great interest to compare the immunological effect of two mouse chows with substantial differences regarding micro-, macronutrient, lipid and vitamin content on the food allergic response in our previously established mouse model. As the two mouse chows of interest, we used a soy containing feed with lower fatty acid (FA) amount (soy-containing feed) and compared it to a soy free mouse chow (soy-free feed) in an established protocol of oral immunizations with Ovalbumin (OVA) under gastric acid suppression. In the animals receiving soy-containing feed, OVA-specific IgE, IgG1, IgG2a antibody levels were significantly elevated and food allergy was evidenced by a drop of body temperature after oral immunizations. In contrast, mice on soy-free diet had significantly higher levels of IL-10 and were protected from food allergy development. In conclusion, soy-containing feed was auxiliary during sensitizations, while soy-free feed supported oral tolerance development and food allergy prevention.
我们的饮食通过支持黏膜屏障和直接影响免疫细胞，对免疫反应有显著影响。因此，比较两种鼠粮对我们先前建立的食物过敏反应的免疫影响非常有趣，这两种鼠粮在微量营养素、宏量营养素、脂质和维生素含量方面存在显著差异。作为两种感兴趣的鼠粮，我们使用了一种含有较低脂肪酸（FA）量的含大豆饲料（含大豆饲料），并在胃酸抑制下用卵清蛋白（OVA）进行口服免疫的既定方案中，将其与不含大豆的鼠粮（无大豆饲料）进行了比较。在接受含大豆饲料的动物中，OVA 特异性 IgE、IgG1 和 IgG2a 抗体水平显著升高，并且在口服免疫后体温下降表明发生了食物过敏。相比之下，食用无大豆饲料的小鼠的 IL-10 水平显著升高，并能预防食物过敏的发生。总之，含大豆的饲料在致敏过程中是辅助性的，而无大豆的饲料则支持口服耐受的发展和食物过敏的预防。
