School of Biological Sciences, University of Ulsan, Ulsan 44610, Korea.
Mol Cells. 2018 Dec 31;41(12):1045-1051. doi: 10.14348/molcells.2018.0129. Epub 2018 Nov 14.
The developmentally regulated GTP binding protein 2 (DRG2) is involved in the control of cell growth and differentiation. Here, we demonstrate that DRG2 regulates microtubule dynamics in HeLa cells. Analysis of live imaging of the plus-ends of microtubules with EB1-EGFP showed that DRG2 deficiency (shDRG2) significantly reduced the growth rate of HeLa cells. Depletion of DRG2 increased 'slow and long-lived' subpopulations, but decreased 'fast and short-lived' subpopulations of microtubules. Microtubule polymerization inhibitor exhibited a reduced response in shDRG2 cells. Using immunoprecipitation, we show that DRG2 interacts with tau, which regulates microtubule polymerization. Collectively, these data demonstrate that DRG2 may aid in affecting microtubule dynamics in HeLa cells.
发育调节鸟嘌呤核苷酸交换因子 2（DRG2）参与细胞生长和分化的调控。在这里，我们证明 DRG2 调节 HeLa 细胞中的微管动力学。用 EB1-EGFP 分析微管正极的实时成像表明，DRG2 缺失（shDRG2）显著降低了 HeLa 细胞的生长速度。DRG2 的耗竭增加了“缓慢且长寿”的微管亚群，但减少了“快速且短命”的微管亚群。微管聚合抑制剂在 shDRG2 细胞中的反应降低。通过免疫沉淀，我们表明 DRG2 与 tau 相互作用，后者调节微管聚合。总之，这些数据表明 DRG2 可能有助于影响 HeLa 细胞中的微管动力学。
