Department of Orthodont, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, 646000, Sichuan, China.
School of Pharmacy, Southwest Medical University, Luzhou, 646000, Sichuan, China.
Biochem Biophys Res Commun. 2018 Dec 9;507(1-4):420-425. doi: 10.1016/j.bbrc.2018.11.054. Epub 2018 Nov 16.
Antibiotics and none-steroidal anti-inflammatory drugs are often taken orally to treat human diseases. The use of these drugs adversely could affect the natural oral microbiota composition and oral immune system. In the meanwhile, it may break the original balance of oral micro-ecosystem. Exploring this change is of great importance to host health.
In this study, we took 20 SD rats and divided them into four groups of five rats each. Each of these groups was administered specified doses of amoxicillin (AMX), ornidazole (ORD), aspirin (ASP), or purified water (CTR), using oral gavage daily for 14 days. High-throughput sequencing was used to investigate the microbiota difference in the four groups of rats once the oral gavage completed. ELISA kit was used to determine IgG and SIgA content, to understand the effect of the drugs on the oral immune system.
We found that oral bacterial composition, IgG and sIgA were significantly affected by the use of these drugs. No matter which medication the rats takes, oral microbiota diversity increase significantly. At the genus level, The Lactobacillaceae, which is essential to the human food digest, raised in the aspirin take group. Staphylococcus and Pasteurella increased in the ornidazole group. Klebsiella, Corynebacterium rose significantly in the amoxicillin group. In normal oral cavity without taking the task medicine, Streptococcus, Pasteurella, and Rothia were in a relatively high abundance. IgG and SIgA content also changed by using these drugs, thus indicating applied those drugs impact of the oral immune system.
Our results indicate that antibiotic and none-steroidal anti-inflammatory drugs could influence the oral microbiota composition, which could also destroy the original oral micro-ecosystem environment. The non-antibiotic drug effect on the oral microbiota and oral immune system similar to the antibiotic drug. All these changes may have a negative influence on host health.
抗生素和非甾体抗炎药通常被口服用于治疗人类疾病。这些药物的使用不当可能会影响天然口腔微生物群落组成和口腔免疫系统。同时，它可能会破坏口腔微生态系统的原有平衡。探索这种变化对宿主健康非常重要。
在这项研究中，我们取 20 只 SD 大鼠，将其分为四组，每组 5 只。这些组分别给予特定剂量的阿莫西林（AMX）、奥硝唑（ORD）、阿司匹林（ASP）或纯化水（CTR），每日经口灌胃 14 天。在口服灌胃完成后，我们使用高通量测序技术来研究四组大鼠的微生物群落差异。使用 ELISA 试剂盒来确定 IgG 和 SIgA 的含量，以了解药物对口腔免疫系统的影响。
我们发现，口腔细菌组成、IgG 和 sIgA 均受到这些药物的显著影响。无论大鼠服用哪种药物，口腔微生物多样性都会显著增加。在属水平上，对人类食物消化至关重要的乳杆菌科在阿司匹林组中升高。在奥硝唑组中，葡萄球菌和巴斯德氏菌增加。在阿莫西林组中，克雷伯氏菌和棒状杆菌显著增加。在正常口腔中，不服用任务药物时，链球菌、巴斯德氏菌和罗氏菌的含量相对较高。IgG 和 SIgA 含量也因使用这些药物而发生变化，从而表明应用这些药物会影响口腔免疫系统。
我们的结果表明，抗生素和非甾体抗炎药会影响口腔微生物群落组成，这也可能破坏口腔微生态系统的原始环境。非抗生素药物对口腔微生物群和口腔免疫系统的影响与抗生素药物相似。所有这些变化都可能对宿主健康产生负面影响。
